NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina
Note that Sample Purification beads are not included in this kit. If beads are required, we recommend ordering NEBNext® Ultra™ II FS DNA Library Prep with Sample Purification Beads (NEB #E6177).
You’ll be thrilled to pieces
Do you need a faster, more reliable solution for DNA fragmentation and library construction? The NEBNext® Ultra™ II FS DNA Library Prep Kit meets the dual challenges of constructing high quality libraries from ever-decreasing input amounts, and scalability of library construction.
The kit includes a new DNA fragmentation reagent, which is also combined with end repair and dA-tailing reagents, enabling these steps to be performed in the same tube, with no clean-ups or sample loss.
You’ll be thrilled to pieces with the result – a reliable, flexible, high-quality library prep that is fast and scalable.
Perform fragmentation, end repair and dA-tailing with a single enzyme mix
Experience reliable fragmentation with a single protocol, regardless of DNA input amount or GC content
Prepare high quality libraries from a wide range of input amounts: 100 pg–500 ng
Generate high yields with increased reaction efficiencies and minimized sample loss
Use with DNA in standard buffers (TE, Tris-HCl) and water
Save time with a streamlined workflow: ~ 2.5 hours, with < 15 minutes hands-on time
Vary incubation time to generate a wide range of insert sizes
The NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina provides a fragmentation system: a fast and reliable solution for DNA fragmentation and library construction. A new DNA fragmentation reagent is combined with end repair and dA-tailing reagents, allowing these steps to be performed in the same tube, with no clean-ups or sample loss. The same fragmentation protocol is used for any input amount (100 pg–500 ng), or GC content. The kit also includes the NEBNext Ultra II Ligation Master Mix for adaptor ligation, and the NEBNext Ultra II Q5® Master Mix for uniform, high-fidelity library amplification. Please note that adaptors and primers are not included in the kit and are available separately.
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This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.
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