NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)
The unique workflow of the NEBNext® Small RNA library prep kits addresses the challenge of minimization of adaptor-dimers while achieving production of high-yield, diverse multiplex libraries in a simple protocol.
This version of the kit is intended for use with your barcode system, and please note that index primers for multiplexing are not included in this kit.
- Minimized adaptor-dimer contamination
- High yields
- Input RNA can be Total RNA
- Suitable for methylated small RNAs (e.g., piRNAs) as well as unmethylated small RNAs
The NEBNext Multiplex Small RNA Library Prep Kit for Illumina (Index Primers 1-48) contains the adaptors, primers, enzymes and buffers required to convert small RNAs into indexed libraries for next-generation sequencing on the Illumina platform. The novel workflow has been optimized to minimize adaptor-dimers, while producing high-yield, high-diversity libraries.
Please note that this kit, the NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible), enables use with your own barcode system. This kit contains adaptors but does not include multiplex primers. If index primers are required, kits NEB #E7300, NEB #E7580 or NEB #E7560 should be ordered instead.
Each kit component must pass rigorous quality control standards, and each set of reagents is functionally validated together by construction and sequencing of a small RNA library on the Illumina sequencing platform.
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.
GenomeWeb article reviews NEBNext Small RNA Kits.
Lot ControlThe lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
The following reagents are supplied with this product:
Store at (°C) Concentration Gel Loading Dye, Blue 25 6 X NEBNext 3´ Ligation Enzyme Mix -20 0 % NEBNext 3´ Ligation Reaction Buffer -20 2 X NEBNext 5´ Ligation Reaction Buffer -20 0 % NEBNext 5´ Ligation Enzyme Mix -20 0 % Murine RNase Inhibitor 0 % LongAmp™ Taq 2X Master Mix -20 2 X NEBNext SR Primer for Illumina -20 0 % Quick-Load pBR322 DNA-MspI Digest -20 50 μg/ml DNA Gel Elution Buffer -20 1 X Linear Acrylamide -20 10 mg/ml TE Buffer -20 0 % Nuclease-free Water -20 0 % NEBNext 5´ SR Adaptor for Illumina -20 0 % NEBNext Index 1 Primer for Illumina -20 0 % NEBNext 3´ SR Adaptor for Illumina -20 0 % NEBNext SR RT Primer for Illumina -20 0 % NEBNext First Strand Synthesis Reaction Buffer -20 5 X ProtoScript II Reverse Transcriptase -20 200,000 units/ml
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