NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)
The unique workflow of the NEBNext® Small RNA library prep kits addresses the challenge of minimization of adaptor-dimers while achieving production of high-yield, diverse multiplex libraries in a simple protocol.
- Minimized adaptor-dimer contamination
- High yields
- Input RNA can be Total RNA
- Suitable for methylated small RNAs (e.g., piRNAs) as well as unmethylated small RNAs
A Breakthrough Method of Small RNA Sample Preparation for Next Gen Sequencing
Tips for Optimizing RNA Inputs in NGS Library Construction
12 Quick Tips for NGS Library Preparation
NEBNext for Core Lab Facilities
The novel NEBNext® Small RNA workflow has been optimized to minimize adaptor-dimers while producing high-yield, high-diversity libraries. The NEBNext® Small RNA Library Prep Set for Illumina® (Set 1) includes adaptors and multiplex primers with 12 indices, to enable multiplexing. Additional index primers for multiplexing are included in kits NEB #E7580 and NEB #E7560.
Each kit component must pass rigorous quality control standards, and each set of reagents is functionally validated together by construction and sequencing of indexed small RNA libraries on the Illumina sequencing platform.
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.
GenomeWeb article reviews NEBNext Small RNA Kits.
Lot ControlThe lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
The following reagents are supplied with this product:
Store at (°C) Concentration Gel Loading Dye, Blue 25 6 X NEBNext 3´ Ligation Enzyme Mix -20 NEBNext 3´ Ligation Reaction Buffer -20 2 X NEBNext 5´ Ligation Reaction Buffer -20 NEBNext 5´ Ligation Enzyme Mix -20 Murine RNase Inhibitor LongAmp™ Taq 2X Master Mix -20 2 X NEBNext SR Primer for Illumina -20 NEBNext Index 1 Primer for Illumina -20 NEBNext Index 2 Primer for Illumina -20 NEBNext Index 3 Primer for Illumina -20 NEBNext Index 4 Primer for Illumina -20 NEBNext Index 5 Primer for Illumina -20 NEBNext Index 6 Primer for Illumina -20 NEBNext Index 7 Primer for Illumina -20 NEBNext Index 8 Primer for Illumina -20 NEBNext Index 9 Primer for Illumina -20 NEBNext Index 10 Primer for Illumina -20 NEBNext Index 11 Primer for Illumina -20 NEBNext Index 12 Primer for Illumina -20 Quick-Load pBR322 DNA-MspI Digest -20 50 μg/ml DNA Gel Elution Buffer -20 1 X Linear Acrylamide -20 10 mg/ml TE Buffer -20 Nuclease-free Water -20 NEBNext 5´ SR Adaptor for Illumina -20 NEBNext 3´ SR Adaptor for Illumina -20 NEBNext SR RT Primer for Illumina -20 NEBNext First Strand Synthesis Reaction Buffer -20 5 X ProtoScript II Reverse Transcriptase -20 200,000 units/ml
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