NEBNext® Single Cell/Low Input cDNA Synthesis and Amplification Module
How low can you go?
NEBNext® is now available for single cell and ultra-low RNA inputs!
This unique cDNA synthesis and amplification workflow meets the demand for a highly sensitive, yet robust method that consistently ultimately enables generation of high quality sequencing data from single cell or ultra-low input RNA.
- Generate the highest yields from single cells, or as little as 2 pg - 200 ng RNA
- Experience unmatched detection of low abundance transcripts
- Rely on consistent transcript detection for a wide range of input amounts and sample types
- Observe uniform transcript coverage, regardless of input amount or sample type
- Use with a variety of DNA inputs, including cultured or primary cells, or total RNA
- Save time with a fast, streamlined workflow, minimal handling steps and hands-on time
Please note that this module contains only reagents for cDNA synthesis and amplification. The NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina® (NEB #E6420) includes reagents for DNA library construction.
The NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module uses a template switching method to generate full length cDNAs directly from single cells or 2 pg – 200 ng RNA. This unique workflow enables generation of high quality sequencing data from a broad range of inputs, and superior transcript detection, while providing reliably consistent performance.
- The highest yields from single cells, or 2 pg - 200 ng RNA
- Input can be cultured or primary cells, or total RNA
- Single-tube protocol from cell lysis to cDNA
- Fast, streamlined, automation-friendly workflow, with minimal handling steps and hands-on time
Download extensive performance data in our technical note.
View extensive performance data i in our technical note.
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact email@example.com for further information.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBNext® Single Cell RT Primer Mix -20 NEBNext® Single Cell RT Buffer -20 NEBNext Template Switching Oligo -20 NEBNext Single Cell RT Enzyme Mix -20 NEBNext Single Cell cDNA PCR Master Mix -20 NEBNext Single Cell cDNA PCR Primer -20 NEBNext Cell Lysis Buffer -20 Murine RNase Inhibitor -20 NEBNext Bead Reconstitution Buffer -20 NEBNext Adaptor Dilution Buffer -20 TE Buffer -20 Nuclease-free Water -20 NEBNext® Ligation Enhancer -20 NEBNext® Ultra™ II Ligation Master Mix -20 NEBNext® Ultra II Q5® Master Mix -20 2 X NEBNext® Ultra™ II FS Enzyme Mix -20 NEBNext® Ultra™ II FS Reaction Buffer -20
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- Sample Preparation for Next Generation Sequencing,
- RNA Library Prep
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