NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads
Ribosomal RNAs (rRNAs) are extremely abundant, constituting 80–90% of total RNA. Efficient removal of rRNA is critical to enable cost-effective sequencing of RNA samples, but this can be especially challenging with low quality RNA (e.g., FFPE RNA) and with low input amounts. The NEBNext® rRNA Depletion Kit employs the efficient RNase H method, as well as complete probe tiling of rRNA, thereby ensuring that even degraded rRNA is hybridized and subsequently removed.
- Suitable for low-quality (e.g., FFPE) or high-quality RNA
- Compatible with a broad range of input amounts: 10 ng - 1 µg
- Fast workflow: 2 hours, with less than 10 minutes hands-on time
- Supplied with Agencourt® RNAClean® XP beads for RNA purification
The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads employs an RNaseH-based method (1), (2) to deplete both cytoplasmic (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochondrial ribosomal RNA (12S rRNA and 16S rRNA) from human, mouse and rat total RNA preparations. This product is suitable for both intact and degraded RNA (e.g., FFPE RNA). The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis applications. For added convenience, this kit contains NEBNext RNA Sample Purification Beads (Agencourt RNAClean XP beads from Beckman Coulter).
For larger volume requirements, customized and bulk packaging is available by purchasing through the Custom Solutions department at NEB. Please contact NEBsolutions@neb.com for further information.
The following reagents are supplied with this product:
Store at (°C) Concentration RNase H Reaction Buffer -20 10 X NEBNext rRNA Depletion Solution -20 NEBNext Probe Hybridization Buffer -20 DNase I Reaction Buffer 10 X DNase I (RNase-free) -20 Nuclease-free Water -20 NEBNext RNase H -20 NEBNext® RNA Sample Purification Beads 4
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