NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads

Catalog #SizeConcentrationPriceQtyAdd to Cart
E6350S6 reactions$340.00Add to Cart
E6350L24 reactions$1,250.00Add to Cart
E6350X96 reactions$4,500.00Add to Cart
  

Description

The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads employs an RNaseH-based method (1), (2) to deplete both cytoplasmic (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochondrial ribosomal RNA (12S rRNA and 16S rRNA) from human, mouse and rat total RNA preparations. This product is suitable for both intact and degraded RNA (e.g. FFPE RNA). The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis applications. For added convenience, this kit contains NEBNext RNA Sample Purification Beads (Agencourt® RNAClean® XP beads from Beckman Coulter).
 
NEBNext rRNA Depletion Kit Workflow Times
rRNA Depletion

Kit Components

The following reagents are supplied with this product:

Store at (°C)Concentration
NEBNext RNase H-20
RNase H Reaction Buffer-2010X
NEBNext rRNA Depletion Solution-20
NEBNext Probe Hybridization Buffer-20
DNase I (RNase-free)-20
DNase I Reaction Buffer-2010X
Nuclease-free Water-20
NEBNext® RNA Sample Purification Beads4

Properties and Usage

Materials Required but not Supplied

  • Magnetic Rack (Alpaqua, Cat. #A001322 or equivalent)
  • Thermal Cycler
  • Agencourt® RNAClean® XP (Beckman Coulter, Inc., Cat. #A63987)

References

  1. Adiconis, X. et al (2013). Comparative analysis of RNA sequencing methods for degraded or low-input samples. Nature Methods. 10, 623-629.
  2. Morlan, J.D. et al. (2012). Selective depletion of RNA enables whole transcriptome profiling of archival fixed tissue. PLos One. 77, e42882.

FAQs

  1. Can I use this product with degraded RNA or fragmented RNA?
  2. What total RNA input should I use?
  3. What is the expected RNA yield recovered after rRNA depletion?
  4. What is the percentage of rRNA left after depletion?
  5. I am currently using RNAClean XP magnetic beads. Will the NEBNext RNA Sample Purification Beads be a suitable replacement?
  6. To remove ribosomal RNA from total RNA, should I use the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) or the NEBNext rRNA Depletion Kit (NEB #E6310)?

Protocols

  1. Protocol for use with NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (E6310, E6350)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.