The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads employs an RNaseH-based method (1), (2) to deplete both cytoplasmic (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochondrial ribosomal RNA (12S rRNA and 16S rRNA) from human, mouse and rat total RNA preparations. This product is suitable for both intact and degraded RNA (e.g. FFPE RNA). The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis applications. For added convenience, this kit contains NEBNext RNA Sample Purification Beads (Agencourt® RNAClean® XP beads from Beckman Coulter).
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| RNase H Reaction Buffer | -20 | 10 X |
| NEBNext rRNA Depletion Solution | -20 | |
| NEBNext Probe Hybridization Buffer | -20 | |
| DNase I Reaction Buffer | -20 | 10 X |
| DNase I (RNase-free) | -20 | |
| Nuclease-free Water | -20 | |
| NEBNext RNase H | -20 | |
| NEBNext® RNA Sample Purification Beads | 4 |
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