The NEBNext End Repair Module has been optimized to convert 1 μg–5 μg of fragmented DNA to blunt-ended DNA having 5′ phosphates, and 3′-hydroxyls. The module is optimized for use with the NEBNext dA-Tailing Module (NEB #E6053), and is part of the original standard DNA library prep workflow for Illumina sequencing.
Each kit component must pass rigorous quality control standards, and for each new lot the entire set of reagents is functionally validated together by construction and sequencing of an indexed library on the Illumina sequencing platform.
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