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  • NEBNext® End Repair Module

    Description

    The NEBNext® End Repair Module has been optimized to repair DNA fragmented by nebulization, acoustic shearing, or nucleases. Fragmented DNA is converted by the NEBNext End Repair Module to blunt ended DNA having 5´-phosphates and 3´-hydroxyl. The NEBNext End Repair Module is provided as a master mix to maximize efficiency and convenience in DNA sample preparation workflows.

    DNA repaired by the NEBNext End Repair Module can be subsequently converted to DNA having 3´ dA-tails with the NEBNext dA-tailing Module or used directly for blunt end cloning or blunt ended adaptor ligation.

    The NEBNext End Repair Module has been validated by sequencing with an Illumina sequencer in conjunction with the NEBNext dA-Tailing Module, NEBNext Quick Ligation Module and Phusion™ High-Fidelity PCR Master Mix, and NEBNEXT High Fidelity 2X PCR Master Mix.

    The NEBNext End Repair Module has also been validated by sequencing with 454 GS FLX Titanium in conjunction with the NEBNext Quick Ligation Module and the NEBNext Adapter Fill-in and ssDNA Isolation Module.

    For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact OEM@neb.com for further information.


    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    NEBNext End Repair Enzyme Mix
    NEBNext End Repair Reaction Buffer10X

    Advantages and Features

    Features

    • Efficient – Converts 1-5 μg fragmented DNA to blunt ended DNA
    • Convenient – Reactions are provided in master mix format to reduce steps during DNA sample prep workflows
    • Automation Friendly

    Applications

    • DNA sample preparation
    • End repair of 1-5 μg fragmented DNA

    Properties and Usage

    Storage Temperature

    -20°C

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Manuals

    The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. What type of sample prep/library construction can I use these reagents for?
    2. How much DNA can I blunt and phosphorylate in one NEBNext End Repair Reaction?
    1. NEBNext End Repair Module Protocol (E6050)