Taq PCR Kit

Description

  
The Taq PCR Kit contains a sufficient supply of recombinant, highly purified Taq DNA Polymerase, PCR-qualified buffer solutions, deoxynucleotides and a broad-range, pre-mixed, ready-to-load DNA marker to perform 200 PCR reactions.

Highlights

Robust and reliable reactions
Tolerates a wide range of templates
Incorporates dUTP, dITP and fluorescently-labeled nucleotides
Exceptional value in terms of cost per unit

Kit Components

The following reagents are supplied with this product:

Store at (°C)Concentration
Deoxynucleotide (dNTP) Solution Mix-2010 mM
Quick-Load® 2-Log DNA Ladder(0.1-10.0 kb)4100 μg/ml
Standard Taq Reaction Buffer Pack-2010X
Standard Taq (Mg-free) Reaction Buffer Pack-2010X
Taq DNA Polymerase with Standard Taq Buffer-205,000 units/ml
Magnesium Chloride (MgCl2) Solution-2025 mM

Properties and Usage

Storage Temperature

-20°C

References

  1. Saiki, R.K. et al. (1985). Science. 230, 1350-1354.
  2. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
  3. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
  4. Lawyer, F.C. et al. (1993). PCR Method and Appl.. 2, 275-287.
  5. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res.. 18, 7317-7322.
  6. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
  7. Powell, L.M. et al. (1987). Cell. 50, 831-840.

Protocols

  1. Protocol for a Routine Taq PCR
  2. Taq DNA Polymerase Guidelines for PCR Optimization

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

Selection Charts

Usage Guidelines & Tips

Troubleshooting Guides

Interactive Tools

Quality Control

Quality Control Assays

The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here.
  • Endonuclease Activity (Nicking):
    The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
  • PCR Amplification (Hot Start, Human Genomic DNA):
    The polymerase is tested in a hot start polymerase chain reaction (PCR) using Human genomic DNA as the control template and specific primers, resulting in an increase in yield of the expected product and a decrease in non-specific genomic bands when compared to a non-hot start control reaction.
  • Single Stranded DNase Activity (FAM Labeled Oligo):
    The product is tested in a reaction containing a fluorescent internal labeled single stranded oligonucleotide. The percent degradation is determined by capillary electrophoresis.

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.