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K. lactis Protein Expression Kit

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

The K. lactis Protein Expression Kit provides an easy method for expressing a gene of interest in the yeast Kluyveromyces lactis. Proteins may be produced intracellularly or secreted using an integrative expression vector (pKLAC2). To achieve protein secretion, a gene of interest is cloned downstream of the K. lactis α-mating factor secretion domain, which is eventually processed in the Golgi and results in secretion of the desired protein.

  • Supplied with competent K. lactis cells (NEB# C1001)
  • Allows cloning and expression of genes toxic to E. coli
  • Rapid high cell density growth results in high yield protein expression 
  • Easy-to-use protocols for those inexperienced with yeast systems
  • No expensive antibiotics or methanol required
  • Attractive commercial sub-licensing
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Catalog # Concentration Size List Price Your Price Quantity
E1000S Not Applicable 1 set $958.00
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