Quick Blunting™ and Quick Ligation™ Kits

Kit Components

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
E0542S     -20       Quick Ligase M2200SVIAL -20 1 x 0.03 ml Not Applicable   Quick Ligation Reaction Buffer B2200SVIAL -20 1 x 1 ml 2 X   Blunting Enzyme Mix M1201AVIAL -20 1 x 0.025 ml Not Applicable   10X Blunting Buffer B1201SVIAL -20 1 x 0.5 ml 10 X   Deoxynucleotide Solution Mix (1 mM) N1201AVIAL -20 1 x 0.1 ml 1 mM

Companion Products

• Quick Blunting™ Kit
• Quick Ligation™ Kit
• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

1. Please note storage conditions for individual products.

1. Blunting protocol for NEB PCR Cloning Kit
2. Protocol for the Quick Blunting Kit (E1201)
3. Transformation Protocol
4. Quick Ligation Protocol (M2200)

• Tips for Maximizing Ligation Efficiencies

• NEBcloner^®
• NEBioCalculator®

1. Will the PreCR Repair Mix blunt the ends of the DNA?

• Troubleshooting Tips for Ligation Reactions

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• E0542S_v0_10009145
• E0542S_v0_10021288
• E0542L_v0_10023229
• E0542S_v0_10037246
• E0542L_v0_10040085
• E0542L_v0_10042834
• E0542S_v0_10045944
• E0542S_v0_10049717
• E0542L_v0_10052507
• E0542S_v0_10057688
• E0542S_v0_10058707
• E0542S_v0_10066882
• E0542L_v0_10065803
• E0542S_v0_10070023
• E0542L_v0_10070595
• E0542S_v0_10093417
• E0542S_v0_10104026
• E0542S_v0_10112467
• E0542S_v0_10122066
• E0542S_v0_10138058
• E0542S_v0_10145591
• E0542S_v0_10152899
• E0542S_v0_10157413
• E0542S_v0_10162755
• E0542S_v0_10167430
• E0542S_v0_10176798
• E0542S_v0_10187344

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Quick Ligation™ Kit
• Quick Ligation Reaction Buffer
• Blunting Enzyme Mix
• 10X Blunting Buffer
• Deoxynucleotide Solution Mix (1 mM)

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.