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  • Quick Blunting™ and Quick Ligation™ Kits

    Description

    NEB offers the Quick Blunting™ and Quick Ligation™ Kits together as a bundle at a value price.

    The Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. DNA is blunted using T4 DNA polymerase (NEB #M0203) which has both 3´→ 5´ exonuclease activity and 5´→ 3´ polymerase activity. T4 Polynucleotide Kinase (NEB #M0201) is included in the enzyme mix for phosphorylation of the 5´ends of blunt-ended DNA for subsequent ligation into a cloning vector. This kit is optimized for blunting up to 5 µg of DNA in a single reaction.

    The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature (25°C).

    Kit Components

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    Quick Ligation Reaction Buffer2X
    10X Blunting Buffer10X
    Deoxynucleotide Solution Mix (1 mM)1 mM
    Quick T4 DNA Ligase-20
    Blunting Enzyme Mix20 reactions

    Properties and Usage

    Notes

    1. Please note storage conditions for individual products.

    FAQs

    1. Will the PreCR Repair Mix blunt the ends of the DNA?

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Usage Guidelines & Tips

    Troubleshooting Guides

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.