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DNA Assembly, Cloning and Mutagenesis Kits

DNA Assembly 

The successful assembly of DNA can be utilized for both cloning and synthetic biology (in which genes and proteins are redesigned and/or assembled into novel ways to create new and useful functionality). New England Biolabs offers several products that can be used for DNA assembly and cloning of multiple DNA fragments, including NEB Gibson Assembly®,  NEBuilder® HiFi DNA Assembly, which utilizes a higher fidelity polymerase, and Golden Gate Assembly. Please refer to the DNA Assembly Selection Chart to determine which product would work best for your needs.

PCR Cloning

PCR cloning is a rapid method for cloning genes, and is often used for projects that require higher throughput than traditional cloning methods can accommodate. It also allows for cloning of fragments that are not available in large amounts. NEB offers an affordable cloning kit as an alternative to TOPO®. The NEB PCR Cloning Kit allows quick and simple cloning of all your PCR amplicons, regardless of the polymerase used. This kit utilizes a novel mechanism for background colony suppression – a toxic minigene is generated when the vector closes upon itself – and allows for direct cloning from your PCR reaction with no purification step. The NEB PCR Cloning Kit is available with and without competent cells.

Site-Directed Mutagenesis

NEB offers the Q5 Site-Directed Mutagenesis Kit as an alternative to QuikChange™. The kit allows for rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The Q5 Site-Directed Mutagenesis Kit is available with and without competent cells.

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Protocols for DNA Assembly, Cloning and Mutagenesis Kits

    Other Tools & Resources


  • DNA Ligase Trifold

    The DNA Ligase Trifold provides information about the extensive selection of DNA ligases and ligases master mixes available from NEB.

  • History of Cloning Poster

    The History of Cloning poster presents some of the major milestones of molecular cloning technologies, in a fun format for your laboratory or classroom.

  • Molecular Cloning Technical Guide

    Download the latest Molecular Cloning Technical Guide for help with product selection, protocols, tips for optimization and trouble-shooting.

  • NEBuilder HiFi DNA Assembly Bifold

    Discover the next generation of gene assembly & cloning, and learn which advantages NEBuilder HiFi delivers over other gene assembly methods.

  • Reagents & Tools for Molecular Cloning brochure

    Learn about recommended products for cloning in our Reagents and Tools for Molecular Cloning Brochure.

  • Synthetic Biology

    Synthetic biology applies the framework of engineering and circuit theory to biological questions. Learn which products and protocols you'll need to get started.

Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

DNA Assembly Selection Chart