FAQ: How long should I incubate cells on ice after DNA has been added (NEB #C2925H and NEB #C2925I)?

Incubating DNA with dam-/dcm- competent cells on ice for 30 minutes is recommended. When tested with pUC19, an incubation time of 20 minutes did not hurt the transformation efficiency (see Figure on the main product page).
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    Tips for Successful Transformations with NEB® Competent Cells

    Follow these tips to get superior results.