Standard Taq (Mg-free) Reaction Buffer Pack

Description

New England Biolabs supplies a unique 10X reaction buffer and a supplemental reagent for use with Taq DNA Polymerase in reaction systems which require a detergent free, low ionic strength buffer and variable magnesium concentration. The Mg-free reaction buffer and supplemental MgCl2 are provided for users that require complete control over Mg2+ concentration to optimize their specific application.

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
Standard Taq (Mg-free) Reaction Buffer-2010X
Magnesium Chloride (MgCl2) Solution-2025 mM

Properties and Usage

Storage Temperature

-20°C

1X Buffer Components

10mM Tris-HCl
50mM KCl
pH 8.3@25°C

FAQs

  1. What is supplied with the Standard Taq (mg-free) Reaction Buffer Pack?
  2. What is the composition of the Standard Taq (mg-free) Reaction Buffer?
  3. The buffer arrived thawed.  Are the buffer and the enzyme still active?
  4. How should the NEBuffer be used?

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Selection Charts

Usage Guidelines & Tips

Interactive Tools

Quality Control

Quality Assurance Statement

  • This reaction buffer and supplement are free of detectable nucleases.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.