• My NEB
  • Print
  • PDF
  • Gel Loading Dye, Blue (6X)


    Gel Loading Dye, Blue (6X) is a pre-mixed loading buffer with one tracking dye for agarose and non-denaturing polyacrylamide gel electrophoresis. This solution contains SDS, which often results in sharper bands, as some restriction enzymes are known to remain bound to DNA following cleavage. EDTA is also included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. Bromophenol blue is the standard tracking dye for electrophoresis. It migrates at approximately 300 bp on a standard 1% TBE agarose gel.

    Properties and Usage

    Storage Temperature


    1X Buffer Components

    2.5% Ficoll®-400
    11mM EDTA
    3.3mM Tris-HCl
    0.017% SDS
    0.015% bromophenol blue
    pH 8.0@25°C

    Quality Control

    Quality Assurance Statement

    • Gel Loading Dye, Blue (6X) is assayed for contaminating endonuclease, exonuclease and RNase activity.


    1. Use 5 µl of Gel Loading Dye, Blue (6X) per 25 µl reaction, or 10 µl per 50 µl reaction. Mix well before loading gel.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.


    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. Are Gel Loading Dye, Blue (6x) and Gel Loading Dye Orange (6x) compatible with other systems such as SYBR (add registered mark symbol) and GelRed during gel electrophoresis?

    Usage Guidelines & Tips