Isothermal Amplification Buffer Pack

Description

10X Isothermal Amplification Buffer is optimized for use with Bst 2.0 DNA Polymerase and Bst 2.0 WarmStart™ DNA Polymerase. This buffer provides superior reaction conditions for isothermal amplication applications.

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
Isothermal Amplification Buffer Pack-2010X

Properties and Usage

Storage Temperature

-20°C

1X Buffer Components

20mM Tris-HCl
10mM (NH4)2SO4
50mM KCl
2mM MgSO4
0.1% Tween® 20
pH 8.8@25°C

Notes

  1. Reaction Conditions:
    At a 1X concentration, the reaction buffer assures optimal activity of Bst 2.0 DNA Polymerase and Bst 2.0 WarmStart DNA Polymerase.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

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Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.