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  • Isothermal Amplification Buffer Pack


    10X Isothermal Amplification Buffer is optimized for use with Bst 2.0 DNA Polymerase and Bst 2.0 WarmStart™ DNA Polymerase. This buffer provides superior reaction conditions for isothermal amplication applications.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    Isothermal Amplification Buffer-2010X

    Properties and Usage

    Storage Temperature


    1X Buffer Components

    20mM Tris-HCl
    10mM (NH4)2SO4
    50mM KCl
    2mM MgSO4
    0.1% Tween® 20
    pH 8.8@25°C


    1. Reaction Conditions:
      At a 1X concentration, the reaction buffer assures optimal activity of Bst 2.0 DNA Polymerase and Bst 2.0 WarmStart DNA Polymerase.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.


    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.