Phusion^® GC Buffer Pack

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
B0519S     -20       Phusion® GC Buffer Pack B0519SVIAL -20 4 x 1.5 ml 5 X   MgCl2 solution B0510AVIAL -20 2 x 1.5 ml 50 mM   DMSO B0515AVIAL -20 1 x 0.5 ml 100 %

Companion Products

• Phusion® HF Buffer Pack
• Phusion® High-Fidelity PCR Kit
• Phusion® High-Fidelity DNA Polymerase
• Phusion® High-Fidelity PCR Master Mix with HF Buffer
• Phusion® High-Fidelity PCR Master Mix with GC Buffer
• Phusion® Hot Start Flex DNA Polymerase
• Phusion® Hot Start Flex 2X Master Mix

• Activity of Restriction Enzymes in PCR Buffers

• PCR Selector
• Tm Calculator

1. What has changed?
2. Have the formulations or any other characteristics of these products changed now that they are manufactured by NEB?
3. Can I continue to use the same reaction conditions if I use Phusion® products with the new product numbers?
4. Are the prices and sizes of these products the same now that they are manufactured by NEB?
5. The buffer arrived thawed.  Are the buffer and the enzyme still active?

• Polymerase Fidelity: What is it, and what does it mean for your PCR?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• B0519S_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• B0519S_v1_0041703
• B0519S_v1_0051804
• B0519S_v1_10009801
• B0519S_v1_10023233
• B0519S_v1_10031987
• B0519S_v1_10033769
• B0519S_v1_10041337
• B0519S_v1_10044649
• B0519S_v1_10047945
• B0519S_v1_10049378
• B0519S_v1_10053460
• B0519S_v1_10060079
• B0519S_v1_10065630
• B0519S_v1_10072488
• B0519S_v1_10081716
• B0519S_v1_10085014
• B0519S_v1_10093305
• B0519S_v1_10097900
• B0519S_v1_10100879
• B0519S_v1_10103221
• B0519S_v1_10104947
• B0519S_v1_10109233
• B0519S_v1_10112810
• B0519S_v1_10116092
• B0519S_v1_10120932
• B0519S_v1_10127945
• B0519S_v1_10132971
• B0519S_v1_10142605
• B0519S_v1_10152513
• B0519S_v1_10158953
• B0519S_v1_10164988
• B0519S_v1_10170519
• B0519S_v1_10175563

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Phusion^® GC Buffer Pack
• MgCl₂ solution
• DMSO

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific.

Notice to purchaser: Limited license.
The purchase price of this product includes a limited, non-transferable license under U.S. and foreign patents owned by BIO-RAD Laboratories, Inc., to use this product. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product.

The purchase price of this product includes a limited, non-transferable license under a patent owned by New England Biolabs, Inc., to use this product. No other license under this patent is conveyed expressly or by implication to the purchaser by the purchase of this product.

PCR license notice: These products are sold under licensing arrangements of Finnzymes Oy with F. Hoffman-La Roche LTD, Roche Molecular Systems, Inc. and Applied Biosystems. The purchase of these products is accompanied by a limited license to use them in the Polymerase Chain Reaction (PCR) process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front fee, either by payment to Applied Biosystems or as purchased, i.e. an authorized thermal cycler.  

Trademarks

PHUSION® is a registered trademark and property of Thermo Fisher Scientific.
THERMO SCIENTIFIC® is a registered trademark of Thermo Fisher Scientific.