Phusion® HF Buffer Pack


New England Biolabs supplies a unique 5X reaction buffer and supplemental reagents for use with Thermo Scientific® Phusion High-Fidelity DNA Polymerase and associated products, all of which are manufactured and quality controlled at New England Biolabs.

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
MgCl2 solution50 mM

Properties and Usage

Storage Temperature



  1. I see foaming when my Phusion® High-Fidelity DNA Polymerase-amplified PCR products are spotted on microarray slides.
  2. What has changed?
  3. Have the formulations or any other characteristics of these products changed now that they are manufactured by NEB?
  4. Can I continue to use the same reaction conditions if I use Phusion® products with the new product numbers?
  5. Are the prices and sizes of these products the same now that they are manufactured by NEB?
  6. The buffer arrived thawed.  Are the buffer and the enzyme still active?

Selection Charts

Feature Articles

Usage Guidelines & Tips

Interactive Tools

Quality Control

Quality Assurance Statement

  • The reaction buffer and supplements are free of detectable nucleases.


The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.