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  • Phusion® HF Buffer Pack


    New England Biolabs supplies a unique 5X reaction buffer and supplemental reagents for use with Thermo Scientific® Phusion High-Fidelity DNA Polymerase and associated products, all of which are manufactured and quality controlled at New England Biolabs.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    MgCl2 solution50 mM

    Properties and Usage

    Storage Temperature


    Quality Control

    Quality Assurance Statement

    • The reaction buffer and supplements are free of detectable nucleases.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.


    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. I see foaming when my Phusion® High-Fidelity DNA Polymerase-amplified PCR products are spotted on microarray slides.
    2. What has changed?
    3. Have the formulations or any other characteristics of these products changed now that they are manufactured by NEB?
    4. Can I continue to use the same reaction conditions if I use Phusion® products with the new product numbers?
    5. Are the prices and sizes of these products the same now that they are manufactured by NEB?
    6. The buffer arrived thawed.  Are the buffer and the enzyme still active?

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