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  • BioBrick® Assembly Kit

    Description

    The BioBrick® Assembly Kit provides a streamlined method for assembly of BioBrick parts into multi-component genetic systems. BioBrick parts are DNA sequences that encode a defined biological function and can be readily assembled with any other BioBrick part. The process for assembling any two BioBrick parts is identical and results in a new composite BioBrick part.

    The BioBrick Assembly Kit contains EcoRI-HF™, XbaI, SpeI, PstI, T4 DNA Ligase and NEBuffer 2.1.

    BioBrick Assembly Overview BioBrick Assembly Overview

    Kit Components

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    XbaI-2020,000 units/ml
    EcoRI-HF®-2020,000 units/ml
    PstI-2020,000 units/ml
    T4 DNA Ligase-20400,000 units/ml
    SpeI-2010,000 units/ml
    NEBuffer 2.1-2010X

    Properties and Usage

    FAQs

    1. I am competing in the iGEM competition. Do you have any products that I should consider purchasing from NEB?

    Protocols

    1. Digestion Protocol for BioBrick Assembly Kit (E0546)
    2. Ligation Protocol using BioBrick Assembly Kit® (E0546)

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Interactive Tools

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.