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NEBNext Ultra II Directional RNA produces the highest yields with FFPE RNA
Dir_RNA_FFPE_Figure1
Ribosomal RNA was depleted from human adult normal liver tissue FFPE Total
RNA (Biochain # R2234149. RIN 2.5) and libraries were made using NEBNext Ultra
II Directional RNA Kit (plus the NEBNext rRNA Depletion Kit (Human/Mouse/Rat)),
Kapa Stranded RNA-Seq Kit with RiboErase, Kapa HyperPrep Kit with RiboErase,
and Illumina TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero™ Gold.
The input RNA amount and number of PCR cycles are indicated. Library yields from
an average of three replicates are shown.

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Available Kits:

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NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®
Includes optimized mixes for directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) for sequencing on the Illumina platform.

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NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads
Includes optimized mixes for directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) plus SPRIselect® beads for size selection and cleanup.