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NEBNext Ultra II Directional RNA produces yields several fold higher than the original Ultra Directional RNA Kit
Poly(A) containing mRNA was isolated from Human Universal Reference RNA (Agilent #740000) using the NEBNext Poly(A) mRNA Magnetic Isolation Module, and libraries were made using either the Ultra II Directional RNA Kit or the original Ultra Directional RNA Kit. Significantly higher yields were achieved with the Ultra II Directional RNA Kit than with the original Ultra Directional RNA Kit. The input RNA amount and number of PCR cycles are indicated. Library yields from an average of three replicates are shown.

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