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NEBNext Ultra II Directional RNA produces yields several fold higher than the original Ultra Directional RNA Kit
Dir_RNA_Yield_Figure5
Ribosomal RNA was depleted from Human Universal Reference RNA (Agilent #740000) using the NEBNext rRNA Depletion Kit (Human/Mouse/Rat) and libraries were made using either the Ultra II Directional RNA Kit or the original Ultra Directional RNA Kit. The input RNA amount and number of PCR cycles are indicated. Significantly higher yields were achieved with the Ultra II Directional RNA Kit than with the original Ultra Directional RNA Kit. Library yields from an average of three replicates are shown.

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Available Kits:

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NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®
Includes optimized mixes for directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) for sequencing on the Illumina platform.

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NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads
Includes optimized mixes for directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) plus SPRIselect® beads for size selection and cleanup.