No. The kit can be used with the NEBNext LV Unique Dual Index Primers. For a complete list of low volume (LV) unique dual index primers, please see the third column of NEBNext® Multiplex Oligos Selection Chart. For use with NEBNext library prep kits, consult the library prep kit manual. The NEBNext LV Unique Dual Index Primers manuals also contain tips for setting up ligation reactions. For color balancing options, please see NEBNext® Index Oligo Selector for valid barcode combinations.
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