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University of Michigan, BSRB

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NEB # DNA Modifying Enzymes and Cloning Technologies Size Stockroom #
E2611S Gibson Assembly® Master Mix 10 reactions E2611S
E2621S NEBuilder® HiFi DNA Assembly Master Mix 10 reactions E2621S
M2200S Quick Ligation™ Kit 30 reactions M2200S
M0202S T4 DNA Ligase 20,000 units M0202S
NEB # Epigenetics Size Stockroom #
R0176S DpnI 1,000 units R0176S
R3138S SalI-HF® 2,000 units R3138S
NEB # Markers & Ladders Size Stockroom #
N3232S 1 kb DNA Ladder 0.2 ml N3232S
N3231S 100 bp DNA Ladder 0.1 ml N3231S
NEB # PCR, Polymerases & Amplification Technologies Size Stockroom #
M0530S Phusion® High-Fidelity DNA Polymerase 100 units M0530S
M0530L Phusion® High-Fidelity DNA Polymerase 500 units M0530L
M0492S Q5® High-Fidelity 2X Master Mix 100 reactions (50 µl vol) M0492S
M0491S Q5® High-Fidelity DNA Polymerase 100 units M0491S
M0267L Taq DNA Polymerase with ThermoPol® Buffer 2,000 units M0267L
NEB # Protein Tools Size Stockroom #
P8107S Proteinase K, Molecular Biology Grade 2 x 1 ml vials P8107S
NEB # Restriction Endonucleases Size Stockroom #
R3552S AgeI-HF® 300 units R3552S
R0558S AscI 500 units R0558S
R0144S BglII 2,000 units R0144S
R0101S EcoRI 10,000 units R0101S
R3101S EcoRI-HF® 10,000 units R3101S
R3195S EcoRV-HF® 4,000 units R3195S
R3104S HindIII-HF® 10,000 units R3104S
R0105S HpaI 500 units R0105S
R3142S KpnI-HF® 4,000 units R3142S
R3589S MfeI-HF® 500 units R3589S
R3131S NheI-HF® 1,000 units R3131S
R3189S NotI-HF® 500 units R3189S
R3189L NotI-HF® 2,500 units R3189L
R0547S PacI 250 units R0547S
R0560S PmeI 500 units R0560S
R3156S SacI-HF® 2,000 units R3156S
R0141S SmaI 2,000 units R0141S
R3133S SpeI-HF® 500 units R3133S
R0145S XbaI 3,000 units R0145S
R0146S XhoI 5,000 units R0146S
R0180S XmaI 500 units R0180S
NEB # RNA Reagents Size Stockroom #
N0447S Deoxynucleotide (dNTP) Solution Mix 8 µmol of each N0447S
M0201S T4 Polynucleotide Kinase 500 units M0201S

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