Time-Saver™ Qualified Enzymes
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Recombinant |  |
Digest in 5 minutes |
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Enzymes that are Time-Saver qualified will digest unit assay substrate in 5-15 minutes under recommended reaction conditions, and can also be used safely in overnight digestions |  |
Digest in 15 minutes |
 |
Not completely digested in 15 minutes | ||
| NT | Not tested. |
| Enzyme | Sequence | Recombinant | Time-Saver Qualified | Unit Assay Substrate | Plasmid Substrate | PCR Substrate |
|---|---|---|---|---|---|---|
| AatII | GACGT/C |  |
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| AccI | GT/MKAC | |
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| Acc65I | G/GTACC | |
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| AciI | CCGC(-3/-1) | |
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| AclI | AA/CGTT | |
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| AcuI | CTGAAG(16/14) | |
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| AflII | C/TTAAG | |
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| AgeI-HF® | A/CCGGT | |
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| AhdI | GACNNN/NNGTC | |
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| AluI | AG/CT | |
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| AlwNI | CAGNNN/CTG | |
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| ApaI | GGGCC/C | |
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| ApaLI | G/TGCAC | |
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| ApeKI | G/CWGC | |
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| ApoI-HF | R/AATTY | |
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| AscI | GG/CGCGCC | |
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NT |
| AseI | AT/TAAT | |
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NT |
| AvaI | C/YCGRG | |
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| AvaII | G/GWCC | |
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| AvrII | C/CTAGG | |
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NT | NT |
| BaeGI | GKGCM/C | |
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| BaeI | (10/15)ACNNNNGTAYC(12/7) | |
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| BamHI § | G/GATCC | |
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| BamHI-HF® | G/GATCC | |
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| BbsI § | GAAGAC(2/6) | |
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| BbsI-HF® | GAAGAC(2/6) | |
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| BbvI | GCAGC(8/12) | |
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| BciVI | GTATCC(6/5) | |
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| BclI § | T/GATCA | |
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| BclI-HF | T/GATCA | |
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| BcoDI | GTCTC(1/5) | |
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| BfuAI | ACCTGC(4/8) | |
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| BglI | GCCNNNN/NGGC | |
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| BglII | A/GATCT | |
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| BlpI | GC/TNAGC | |
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| BmgBI | CACGTC(-3/-3) | |
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| BmtI-HF® | GCTAG/C | |
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| BpuEI | CTTGAG(16/14) | |
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| BsaAI | YAC/GTR | |
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| BsaHI | GR/CGYC | |
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| BsaI-HF®v2 | GGTCTC(1/5) | |
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| BsaWI | W/CCGGW | |
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| BsaXI | (9/12)ACNNNNNCTCC(10/7) | |
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| BseRI | GAGGAG(10/8) | |
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| BsgI | GTGCAG(16/14) | |
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| BsiEI | CGRY/CG | |
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| BsiHKAI | GWGCW/C | |
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| BsiWI § | C/GTACG | |
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| BsiWI-HF® | C/GTACG | |
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| BslI | CCNNNNN/NNGG | |
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| BsmAI | GTCTC(1/5) | |
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| BsmBI-v2 | CGTCTC | |
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| BsmI | GAATGC(1/-1) | |
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| BsoBI | C/YCGRG | |
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| BspCNI | CTCAG(9/7) | |
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| BspEI | T/CCGGA | |
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| BspHI | T/CATGA | |
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| Bsp1286I | GDGCH/C | |
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| BspQI | GCTCTTC(1/4) | |
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| BsrBI | CCGCTC(-3/-3) | |
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| BsrDI | GCAATG(2/0) | |
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| BsrFI-v2 | R/CCGGY | |
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| BsrGI-HF® | T/GTACA | |
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| BsrI | ACTGG(1/-1) | |
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| BssHII | G/CGCGC | |
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| BssSI-v2 | CACGAG(-5/-1) | |
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| BstBI | TT/CGAA | |
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| BstEII-HF® | G/GTNACC | |
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| BstNI | CC/WGG | |
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| BstUI | CG/CG | |
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| BstXI | CCANNNNN/NTGG | |
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| BstYI | R/GATCY | |
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| BstZ17I-HF® | GTATAC | |
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| Bsu36I | CC/TNAGG | |
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| BtgI | C/CRYGG | |
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| BtsCI | GGATG(2/0) | |
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| BtsI-v2 | GCAGTG(2/0) | |
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| Cac8I | GCN/NGC | |
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| ClaI | AT/CGAT | |
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| CspCI | (11/13)CAANNNNNGTGG(12/10) | |
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| CviQI | G/TAC | |
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| DdeI | C/TNAG | |
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| DpnI | GA/TC | |
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| DpnII | /GATC | |
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| DraI | TTT/AAA | |
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| DraIII-HF® | CACNNN/GTG | |
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| DrdI | GACNNNN/NNGTC | |
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| EagI-HF® | C/GGCCG | |
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| EarI | CTCTTC(1/4) | |
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| Eco53kI | GAG/CTC | |
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| EcoNI | CCTNN/NNNAGG | |
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| EcoO109I | RG/GNCCY | |
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| EcoP15I | CAGCAG(25/27) | |
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| EcoRI § | G/AATTC | |
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| EcoRI-HF® | G/AATTC | |
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| EcoRV § | GAT/ATC | |
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| EcoRV-HF® | GAT/ATC | |
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| Esp3I | CGTCTC(1/5) | |
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| Fnu4HI | GC/NGC | |
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| FseI | GGCCGG/CC | |
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| FspI | TGC/GCA | |
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| HaeII | RGCGC/Y | |
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| HaeIII | GG/CC | |
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| HhaI | GCG/C | |
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| HincII | GTY/RAC | |
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| HindIII-HF® | A/AGCTT | |
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| HinfI | G/ANTC | |
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| HinP1I | G/CGC | |
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| HpaII | C/CGG | |
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| HphI | GGTGA(8/7) | |
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| HpyAV | CCTTC(6/5) | |
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NT |
| HpyCH4IV | A/CGT | |
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| HpyCH4V | TG/CA | |
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| Hpy166II | GTN/NAC | |
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| KpnI-HF® | GGTAC/C | |
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| MboI | /GATC | |
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| MboII | GAAGA(8/7) | |
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| MfeI-HF® | C/AATTG | |
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| MluCI | /AATT | |
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| MluI-HF® | A/CGCGT | |
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| MlyI | GAGTC(5/5) | |
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| MmeI | TCCRAC(20/18) | |
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| MnlI | CCTC(7/6) | |
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| MseI | T/TAA | |
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| MslI | CAYNN/NNRTG | |
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| MspA1I | CMG/CKG | |
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| MspI | C/CGG | |
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| MwoI | GCNNNNN/NNGC | |
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| NciI | CC/SGG | |
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| NcoI § | C/CATGG | |
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| NcoI-HF® | C/CATGG | |
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| NdeI | CA/TATG | |
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| NgoMIV | G/CCGGC | |
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| NheI-HF® | G/CTAGC | |
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| NlaIII | CATG/ | |
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| NotI § | GC/GGCCGC | |
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| NotI-HF® | GC/GGCCGC | |
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| NruI-HF® | TCG/CGA | |
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| NsiI § | ATGCA/T | |
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| NsiI-HF® | ATGCA/T | |
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| NspI | RCATG/Y | |
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| PacI | TTAAT/TAA | |
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| PaeR7I | C/TCGAG | |
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| PflFI | GACN/NNGTC | |
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| PflMI | CCANNNN/NTGG | |
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| PmeI | GTTT/AAAC | |
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NT |
| PmlI | CAC/GTG | |
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| PpuMI | RG/GWCCY | |
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| PshAI | GACNN/NNGTC | |
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| PsiI-v2 | TTA/TAA | |
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| PstI § | CTGCA/G | |
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| PstI-HF® | CTGCA/G | |
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| PvuI-HF® | CGAT/CG | |
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| PvuII § | CAG/CTG | |
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| PvuII-HF® | CAG/CTG | |
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| RsaI | GT/AC | |
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| SacI-HF® | GAGCT/C | |
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| SacII | CCGC/GG | |
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| SalI § | G/TCGAC | |
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| SalI-HF® | G/TCGAC | |
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| SapI | GCTCTTC(1/4) | |
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| SbfI-HF® | CCTGCA/GG | |
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| ScaI-HF® | AGT/ACT | |
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| SfiI | GGCCNNNN/NGGCC | |
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| SfoI | GGC/GCC | |
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| SmaI | CCC/GGG | |
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| SpeI-HF® | A/CTAGT | |
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| SphI-HF® | GCATG/C | |
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| SrfI | GCCC/GGGC | |
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| SspI-HF® | AAT/ATT | |
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| StuI | AGG/CCT | |
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| StyD4I | /CCNGG | |
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| StyI-HF® | C/CWWGG | |
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| SwaI | ATTT/AAAT | |
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| TaqI-v2 | T/CGA | |
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| TfiI | G/AWTC | |
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| TseI | G/CWGC | |
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| TspMI | C/CCGGG | |
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| TspRI | NNCASTGNN/ | |
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| Tth111I | GACN/NNGTC | |
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| XbaI | T/CTAGA | |
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| XhoI | C/TCGAG | |
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| XmaI | C/CCGGG | |
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| XmnI | GAANN/NNTTC | |
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§ An HF version of this enzyme is available.
