DNA Ligase Selection Chart

While more than one ligase may work for your application, the following selection chart presents our recommendations for optimal performance. To select a ligase based upon the type of substrate present, please visit Substrate-based Ligase Selection Chart. In addition, the Properties of DNA and RNA Ligaseswill help you understand the differences between them.

	Blunt/TA Ligase Master Mix	Instant Sticky-end Master Mix	ElectroLigase®	T4 DNA Ligase	Hi-T4™ DNA Ligase	Immobilized T4 DNA Ligase	Salt-T4^® DNA Ligase	Quick Ligation™ Kit	NEBridge^® Ligase Master Mix	T3 DNA Ligase	T7 DNA Ligase	HiFi Taq DNA Ligase	Taq DNA Ligase	9°N™ DNA Ligase	NEBNext Quick Ligation™ Module	SplintR® Ligase	E.coli DNA Ligase
DNA APPLICATIONS
Ligation of sticky ends	★★	★★★	★★	★★	★★	★★★	★★	★★★	★	★★	★★	★	★	★			★
Ligation of blunt ends	★★★	★	★★	★★	★★	★	★★	★★★	★	★★
T/A cloning	★★★	★	★★	★★	★★		★★	★★		★	★
Electroporation			★★★	★★	★★
Golden Gate Assembly				★★★	★★*				★★★		★
Ligation of sticky ends only											★★★
Repair of nicks in dsDNA	★★	★★	★★	★★★	★★★		★★★	★★		★★	★★	★★	★★	★★		★★	★★
High-complexity library cloning	★★	★★	★★	★★★				★★
Adapter Ligation ▲	★★★	★★	★★	★		▲		★★		★					▲
Ligation-Dependent DNA Sequence & SNP Detection (LCR, LDR & related methods)												★★★	★★	★★
Ligation-Dependent RNA Sequence & SNP Detection				★												★★★
Ligation of adjacent ssDNAs on an RNA splint																★★★
NGS APPLICATIONS
NGS Library Prep dsDNA-dsDNA (ligation)	▲			▲						▲					▲
FEATURES
Salt tolerance (>2X that for T4 DNA Ligase)							✓			✓
Ligation in 15 min. or less	✓	✓		✓	✓		✓	✓		✓	✓	✓	✓	✓	✓	✓
Master Mix Formulation	✓	✓							✓						✓
Thermostable												✓	✓	✓
Thermotolerant					✓							✓	✓	✓
Recombinant	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓
Reusable/Removable						✓

KEY
★★★	Optimal, recommended ligase for selected application
★★	Works well for selected application
★	Will perform selected application, but is not recommended
▲	Please consult the specific NGS protocol to determine the optimal enzyme for your needs

*An efficient cloning method to expand vector and restriction site compatibility of Golden Gate Assembly

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