New England Biolabs has an extensive offering of enzymes that modify RNA. RNA modifying enzymes are used to prepare RNA for cloning (NEB #M0290, NEB #M0201, NEB #M0356), for direct RNA labeling (NEB #M0201, NEB #M0228), and for RNA modification before transfection (NEB #M2080, NEB #M0366), among numerous other applications.
- A Typical Deadenylation Reaction (M0331)
- Capping Protocol (M2080)
- End-labeling Protocol
- Non-radioactive Phosphorylation with T4 PNK or T4 PNK (3´ phosphatase minus)
- A Typical Tailing Reaction (M0337)
- Protocol for Dephosphorylation of 5'-ends of DNA using CIP (NEB #M0290)
- Protocol for Dephosphorylation of 5´-ends of DNA using Antarctic Phosphatase (NEB #M0289)
- Radioactive Labeling with T4 PNK or T4 PNK (3´ phosphatase minus)
- 2´-O-Methylation of Capped RNA (M0366)
- One-Step Capping and 2´-O-Methylation (M0366)
- Labeling Protocol (M2080)
- Protocol for Dephosphorylation of 5´-ends of DNA using rSAP (NEB #M0371)
- Poly(A) Tailing of RNA using E. coli Poly(A) Polymerase (NEB# M0276)
- Ligation of a DNA or RNA oligo to single stranded RNA using T4 RNA Ligase 1
- Decapping eukaryotic mRNA with RppH (NEB #M0356)
- Protocol for Dephosphorylation of 5´-ends of DNA using Quick Dephosphorylation Kit (M0508)
- Enzymatic PCR Cleanup Protocol (NEB #M0371)
- Avoiding Ribonuclease Contamination
- Wulf, M.G., Maguire, S., Humbert, P., Dai, N., Bei, Y., Nichols, N.M., Correa, I.R., Jr., Guan, S (2019) Non templated addition and template switching by Moloney murine leukemia virus MMLV based reverse transcriptases co occur and compete with each other J Biol Chem; 294(48), 18220-18231.. PubMedID: 31640989, DOI: 10.1074/jbc.RA119.010676
- Wulf, Madalee; Buswell, John; Chan, Siuhong; Dai, Nan; Marks, Katherine; Tzertzinis, George; Whipple, Joe; Correa, Ivan; Schildkraut, Ira; (2019) The yeast scavenger decapping enzyme DcpS and its application for in vitro RNA recapping Sci Rep; 9 (1), 8594. PubMedID: 31197197, DOI: 10.1038/s41598-019-45083-5
- Eyler, D.E., Franco, M.K., Batool, Z., Wu, M.Z., Dubuke, M.L., Dobosz-Bartoszek, M., Jones, J.D., Polikanov, Y.S., Roy, B., Koutmou, K.S (2019) Pseudouridinylatio of mRNA coding sequences alters translation Proc Natl Acad Sci U S A; 116(46), 23068-23074.. PubMedID: 31672910, DOI: 10.1073/pnas.1821754116
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