
Proteases
NEB offers several proteases with different recognition sites.
Modified Trypsin (TPCK-treated) (NEB #P8101) selectively cleaves peptide bonds C-terminal to lysine and arginine residues (1). Endoproteinase AspN (NEB #P8104) (flavastacin) and Endoproteinase GluC (NEB #P8100) (Staphylococcus aureus Protease V8) selectively cleave peptide bonds N-terminal to aspartic acid residues and C-terminal to glutamic acid, respectively. These proteases are ideal for proteome analysis by mass spectrophotometry. Proteinase K (NEB #P8102) is a non-specific, subtilisin-related serine protease with a very high specific activity. Furin (NEB #P8077) is a ubiquitous subtilisin-like proprotein convertase with a minimal cleavage site requirement of Arg-X-X-Arg'.
We also offer several proteases with unique, specific recognition sites. Enterokinase (NEB #P8070) is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. Factor Xa (NEB #P8010) cleaves after the arginine residue in its preferred cleavage site Ile-(Glu or Asp)-Gly-Arg. Genenase™ I (NEB #P8075) is a variant of subtilisin BPN´ that has been engineered to have increased specificity by substituting amino acids in its active site (2,3). NEB recommends using Genenase I to cleave fusion proteins at the site Pro-Gly-Ala-Ala-His-Tyr. These proteases can be used with the pMAL™ protein fusion and purification system (NEB #E8200) during the purification step.
(1) Northrop, J.H. and Kunitz, M. (1931) Science, 73, 262-263. PMID: 17755302
(2) Carter, P. and Wells, J.A. (1987) Science, 237, 394-399. PMID: 3299704
(3) Carter, P. et al. (1991) Biochemistry, 30, 6142-6148. PMID: 2059622
Genenase™ I is a trademark of Genencor International Inc.
pMAL™ is a trademark of New England Biolabs, Inc.
Choose Product:
Choose Type:
- Protocol using Trypsin-ultra™, Mass Spectrometry Grade (P8101)
- General protocol to release nucleic acids prior to capillary or gel electrophoresis using Proteinase K (P8107)
- Protocol to cleanup DNA Glucosylation/restriction digest and Proteinase using Proteinase K (P8107)
- Protocol to purify PCR products in preparation for cloning using Proteinase K (P8107)
- Trypsin Digestion Protocol using NEB Trypsin-ultra™ and the FASP Kit
- Using recombinant Cas9 nuclease to assess locus modification in genome editing experiments (#M0386)
- Reaction Conditions for IdeZ Protease (IgG-specific) (P0770)
- Reaction conditions for Simultaneous Digestion of IgG with IdeZ Protease (IgG-specific) and PNGase F (fragmentation and deglycosylation) (P0770)
- In-gel Digestion Protocol for Endoproteinase LysC (P8109)
- Typical Reaction Conditions for Endoproteinase LysC
- Typical GluC Digest Reaction Conditions (P8100)
- Typical GluC In-Gel Digest Reaction Conditions (P8100)
- Typical Trypsin and GluC Co-Digest Reaction Conditions (P8100)
- Glycoproteomics Brochure
Brochures
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly. If you have any questions, please contact Customer Support at freezers@neb.com or 1-800-632-5227 x 8.