Nucleotide Solutions
New England Biolabs supplies a selection of highly pure nucleotides for use with DNA and RNA polymerases.
Deoxynucleotide Solution Set (NEB #N0446)
The Deoxynucleotide Solution Set contains four separate 100 mM solutions of ultrapure nucleotides (dATP, dCTP, dGTP, and dTTP).
Deoxynucleotide Solution Mix (NEB #N0447)
The Deoxynucleotide Solution Mix is an equimolar mixture of ultrapure dATP, dCTP, dGTP, and dTTP. Each nucleotide is present at a concentration of 10 mM in the mixture for a total dNTP concentration of 40 mM.
GMP-grade reagent also available. Learn more.
7-deaza-dGTP* (NEB #N0445)
7-deaza-dGTP is a useful additive for the PCR of GC-rich templates; contains a 5 mM solution of 7-deaza-dGTP as a dilithium salt.
* licensed from Roche Diagnostics GmbH
Acyclonucleotide Set (NEB #N0460)
The Acyclonucleotide Set contains four separate tubes of acyNTPs (acyATP, acyCTP, acyGTP and acyTTP), supplied as a dry powder.
dATP Solution (NEB #N0440)
The dATP solution contains 0.25 ml of 100 mM ultrapure dATP.
Ribonucleotide Solution Set (NEB #N0450)
Ribonucleotide Solution Set consists of four separate 100 mM solutions of ATP, GTP, CTP and UTP.
Ribonucleotide Solution Mix (NEB #N0466)
The Ribonucleotide Solution Mix is an equimolar mixture of ribonucleotide triphosphates (rATP, rCTP, rGTP and rUTP). Each is supplied at a concentration of 25 mM for a total concentration of 100 mM.
Choose Product:
Choose Type:
- How much is in each vial of the Deoxynucleotide Solution Set?
- What is supplied with the Deoxynucleotide Solution Set?
- How does this product differ from the Deoxynucleotide Solutions Set (NEB# N0446)?
- What is supplied with the Deoxynucleotide Solution Mix?
- What is in the Deoxynucleotide Solution Mix?
- What is 7-deaza-dGTP used for?
- Polymerases and Amplification FAQs
-
Understanding Variability in DNA Amplification Reactions
-
DNA Damage - the major cause of missing pieces from the DNA puzzle
-
Anatomy of a Polymerase - How Function and Structure are Related
Read about the relationship between Polymerase structure and function when copying DNA.
- PCR Reagents Brochure
- DNA Polymerase Selection Chart
- PCR Troubleshooting Guide
- DNA Damage and PreCR
- Guidelines for PCR Optimization with Taq DNA Polymerase
Feature Articles
Brochures
Selection Tools
Troubleshooting Guides
Usage Guidelines
Template/product specificity: Is RNA or DNA involved? Is the 3´ terminus at a gap, nick or at the end of the template?
Removal of existing nucleotides: Will the nucleotide(s) be removed from the existing polynucleotide chain as part of the protocol? If so, will they be removed from the 5´ or the 3´ end?
Thermal stability: Does the polymerase need to survive incubation at high temperature or is heat inactivation desirable?
Fidelity: Will subsequent sequence analysis or expression depend on the fidelity of the synthesized products?
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly. If you have any questions, please contact Customer Support at freezers@neb.com or 1-800-632-5227 x 8.
