IdeZ Protease (IgG-specific)

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
P0770S     -20       IdeZ Protease (IgG-specific) P0770SVIAL -20 1 x 0.05 ml 80,000 units/ml   GlycoBuffer 2 B3704SVIAL -20 1 x 1 ml 10 X

Companion Products

• Rapid PNGase F
• Endo S
• Trypsin-ultra™, Mass Spectrometry Grade
• Rapid PNGase F Antibody Standard
• Rapid™ PNGase F (non-reducing format)

1. Store at -20°C for up to two years.
   
2. IdeZ Protease efficiently cleaves human, humanized, chimeric, sheep,rabbit and monkey IgG as well as mouse IgG2a and IgG3. IdeZ Proteasewill also cleave Fc-fusion proteins, such as Enbrel.
3. IdeZ Protease does not cleave mouse IgG1 or IgG2b, rat, porcine, bovineor goat IgG. It also does not cleave non-IgG isotypes including IgA,IgM, IgD and IgE.

1. Reaction Conditions for IdeZ Protease (IgG-specific) (P0770)
2. Reaction conditions for Simultaneous Digestion of IgG with IdeZ Protease (IgG-specific) and PNGase F (fragmentation and deglycosylation) (P0770)

1. What is the advantage of IdeZ over IdeS?
2. Is IdeZ Protease active in Phosphate Buffered Saline (PBS)?
3. Can protein A magnetic beads (NEB# S1425S) be used to create Fc and Fab fragment pools after cleavage with IdeZ Protease?
4. Can IdeZ Protease cleave IgGs other than human?
5. Can IdeZ Protease cleave Ig isotypes other than IgG?
6. Can PNGase F be used together with IdeZ Protease under native conditions to deglycosylate the Fc portion of an antibody?
7. What is the cleavage site for IdeZ Protease (IgG-specific)?

• Glycoproteomics Brochure
• Glycoproteomics Technical Guide
• Purification Beads, Columns and Resins Brochure

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• P0770S_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• P0770S_v1_0031709
• P0770S_v1_0031601
• P0770S_v1_0031607
• P0770S_v1_0031611
• P0770S_v1_0031703
• P0770S_v1_10013498
• P0770S_v1_10036888
• P0770S_v1_10060294
• P0770S_v1_10079718
• P0770S_v1_10089223
• P0770S_v1_10113717
• P0770S_v1_10137585
• P0770S_v1_10161211
• P0770S_v1_10183737
• P0770S_v1_10232776
• P0770S_v1_10248743

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• IdeZ Protease (IgG-specific)
• GlycoBuffer 2

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.