DNA Gel Extraction
Monarch DNA Gel Extraction Kits provide fast and reliable purification, reduced hands-on time and elution in low volumes
Our unique column design offers several improvements over other commercially available products, including the elimination of buffer retention and elution in as little as 6 µl
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FAQs for DNA Gel Extraction
- Do you have any recommendations for purification of ssDNA?
- Can the Monarch PCR & DNA Cleanup Kit (5 μg) be used to purify RNA?
- What is the composition of each buffer provided with the Monarch PCR & DNA Cleanup Kit (5 μg)?
- What factors affect my (A260/A230)?
- Can I excise a fragment from a gel and store it for purification at a later time?
- Are the columns in the Monarch PCR & DNA Cleanup Kit (5 μg) the same as the ones in the Monarch DNA Gel Extraction Kit?
- Are Monarch spin columns compatible with Vacuum Manifolds?
- What type of agarose gels are compatible with the Monarch DNA Gel Extraction Kit?
- What size primers can be effectively removed from a PCR reaction?
- What size of DNA can be purified with the Monarch DNA Cleanup Columns?
- What is the maximum binding capacity of the Monarch DNA Cleanup Column provided with the Monarch PCR & DNA Cleanup Kit (5 μg)?
- After purification, I see a faint additional band running below the expected size on a gel. What happened?
- What is the maximum binding capacity of the Monarch DNA Cleanup Column provided in the Monarch DNA Gel Extraction Kit?
- What is the smallest volume of elution buffer that can be used with the Monarch DNA Cleanup Column?
- Can I use water to elute the DNA when using the Monarch Kits?
- Can ligation and other DNA manipulations be carried out on β-Agarase I treated gel slices containing DNA?
- How stable is β-Agarase I in reaction?
- What is the molecular weight of β-Agarase I?
- What is the optimal pH for β-Agarase I digestion?
- What is a common cause of β-Agarase I reaction failure?
- What type of agarose will β-Agarase I digest?
- Can a high percentage low melt gel be digested with β-Agarase I?
- Why does a white precipitate form after the reaction using β-Agarase I?
- How can β-Agarase I be heat inactivated?
- Does the gel running buffer have an effect on the β-Agarase I reaction?
- Can I use the Monarch DNA & PCR Cleanup Kit to purify oligonucleotides and other short DNA fragments?
Protocols for DNA Gel Extraction
- Oligonucleotide Cleanup Using Monarch® PCR & DNA Cleanup Kit (5 μg) Protocol (NEB #T1030)
- Monarch® DNA Gel Extraction Kit Protocol (NEB #T1020)
- Protocol for DNA Cleanup and Concentration Using the Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB #T1030)
- DNA Purification from Agarose Gels using Beta Agarase I (NEB #M0392)
- Monarch Nucleic Acid Purification Brochure
- Troubleshooting Guide for DNA Cleanup and Plasmid Purification
- Back to basics: Important things to keep in mind when purifying plasmids and DNA fragments
- Six Tips for a Perfect Gel Extraction
Tools & Resources
Brochures
Troubleshooting Guides
Usage Guidelines
Product Manual
Advantages of the Monarch DNA Gel Extraction Kit
- Elute in as little as 6 μl
- Prevent buffer retention and salt carryover with optimized column design
- Save time with fast, user-friendly protocols
- No need to monitor pH or add isopropanol
- Buffers and columns available separately
- Significantly less plastic used when compared with other kits
- Responsibly-sourced and recyclable packaging
Specifications of the Monarch DNA Gel Extraction Kit
- Binding Capacity: up to 5 μg
- DNA Size Range: ~50 bp to 25 kb
- Typical Recovery:
DNA (50 bp to 10 kb): 70–90%
DNA (11–25 kb): 50–70% - Elution Volume: ≥ 6 μl
- Purity: A260/280 ≥ 1.8
- Protocol Time: 10 min of spin and incubation time.
- Compatible Downstream Applications: ligation, restriction digestion, labeling and other enzymatic manipulations, library construction and DNA sequencing
Legal Information
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
