Thermolabile Exonuclease I
neb31 recombinant 37 80 Heat

Interested in a 5-minute protocol? Try out the Exo-CIP Rapid PCR Cleanup Kit (NEB #E1050)

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at

  • DNA specific exonuclease
  • Catalyzes the removal of nucleotides from linear single-stranded DNA in the 3' to 5' direction
  • Unlike Exonuclease I (NEB #M0293), Thermolabile Exonuclease I can be heat inactivated at 80°C in 1 minute 
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

Thermolabile Exonuclease I is ideal for:

  • Removal of single-stranded primers in PCR reactions prior to DNA sequencing or SNP analysis
  • Removal of single-stranded primers for nested PCR reactions
  • Removal of linear single-stranded DNA, leaving behind double-stranded DNA in the sample
Catalog # Concentration Size List Price Your Price Quantity
M0568S 20,000 units/ml 3,000 units $85.00
M0568L 20,000 units/ml 15,000 units $347.00
Please enter a quantity for at least one size

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