HomeFAQsCan Thermolabile Exonuclease I remove primers in nested PCR reactions?
FAQ: Can Thermolabile Exonuclease I remove primers in nested PCR reactions?
Yes, add 1 µl of Thermolabile Exonuclease I to first round of PCR products (up to 20 µl) and incubate the reaction at 37°C for 10 minutes, followed by 80oC for 1 min to inactivate the enzyme. New sets of primers can then be added followed by a second round of PCR to reduce the nonspecific PCR amplicons.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
