Therminator™ DNA Polymerase

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0261S     -20       Therminator™ DNA Polymerase M0261SVIAL -20 1 x 0.1 ml 2,000 units/ml   ThermoPol® Reaction Buffer B9004SVIAL -20 1 x 1.5 ml 10 X
M0261L     -20       Therminator™ DNA Polymerase M0261LVIAL -20 1 x 0.5 ml 2,000 units/ml   ThermoPol® Reaction Buffer B9004SVIAL -20 3 x 1.5 ml 10 X

Application Features

• DNA sequencing by partial ribosubstitution (3)
• DNA sequencing using dideoxy (4) or acyclo (5) chain terminators
• SNP analysis with dideoxy or acyclo chain terminators (6)

Companion Products

• Acyclonucleotide Set
• Deoxynucleotide (dNTP) Solution Mix
• Deoxynucleotide (dNTP) Solution Set

Materials Sold Separately

• ThermoPol® Reaction Buffer Pack
• Magnesium Sulfate (MgSO₄) Solution

1. Amplification of extended regions may require optimization of reaction conditions.

1. Gardner, A.F. and Jack, W.E. (1999). Nucl. Acids Res.. 27, 2545-2555.
2. Gardner, A.F. and Jack, W.E. (2002). Nucl. Acids Res. 30, 605-613.
3. Barnes, W.F. (1978). J. Mol. Biol.. 119, 83-99.
4. Sanger, F., Nicklen, S. and Coulson, A.R. (1977). Proc. Natl. Acad. Sci. USA. 74, 5463-5467.
5. Trainor, G.L. (1996). U.S. Patent No. 5,558,991.
6. Haff, L.A. and Simirnov, I.P. (1997). Genome Methods. 7, 378-388.

• Guidelines for PCR Optimization with Thermophilic DNA Polymerases

• DNA Polymerase Selection Chart

1. Can Therminator™ DNA Polymerase be used in other buffers?
2. When should Therminator™ DNA Polymerase be used?
3. Can Therminator™ DNA Polymerase be substituted for ThermoSequenase™?
4. Can Therminator™ DNA Polymerase be used for sequencing?
5. Why are some of the A's in my sequence weak when using Therminator™ DNA Polymerase?

• PCR Troubleshooting Guide

• Gardner, A.F., Jackson, K.M., Boyle, M.M., Buss, J.A., Potapov, V., Gehring, A.M., Zatopek, K.M., Correa, I.R., Jr., Ong, J.L., Jack. W.E. (2019) Therminator DNA polymerase: Modified nucleotides and unnatural substrates Front Mol Biosci; 6, 28. PubMedID: 31069234, DOI: 10.3389/fmolb.2019.00028

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0261S_L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0261S_L_v1_0201508
• M0261S_L_v1_0201603
• M0261S_L_v1_0201609
• M0261S_L_v1_0201703
• M0261S_L_v1_0201709
• M0261S_L_v1_0201711
• M0261S_L_v1_0201803
• M0261S_L_v1_0211803
• M0261L_v1_10016088
• M0261S_v1_10016089
• M0261L_v1_10026392
• M0261S_v1_10026393
• M0261S_v1_10042500
• M0261L_v1_10050518
• M0261L_v1_10058063
• M0261S_v1_10058064
• M0261L_v1_10062089
• M0261S_v2_10071264
• M0261L_v2_10074208
• M0261S_v2_10101720
• M0261L_v2_10102883
• M0261L_v2_10117204
• M0261L_v2_10134455
• M0261S_v2_10115533
• M0261S_v2_10137267
• M0261S_v2_10155758
• M0261S_v2_10170516
• M0261L_v2_10174177
• M0261L_v2_10191362
• M0261S_v2_10200477
• M0261L_v2_10206685
• M0261S_v2_10219886
• M0261L_v2_10234093
• M0261L_v2_10238411
• M0261S_v2_10246056
• M0261S_v2_10270416
• M0261L_v2_10284536
• M0261L_v2_10298614
• M0261S_v2_10301431
• M0261S_v2_10320063
• M0261L_v2_10325225

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Therminator™ DNA Polymerase
• ThermoPol^® Reaction Buffer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Trademarks

NEW ENGLAND BIOLABS® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.
9°N™ and THERMINATOR™ are trademarks of New England Biolabs, Inc.

SEQUENASE™ is a trademark of GE Healthcare.