Home DNA Modifying Enzymes and Cloning Technologies Products BamHI Methyltransferase

BamHI Methyltransferase
NEBU cloned at NEB recombinant 37 No SAM

The large size of this product was discontinued on 12/31/2020. The small size will continue to be available.

M0223BamHIMethyl_thumb

BamHI Methyltransferase modifies the internal cytosine residue (N4) of the sequence GGATCC.
This enzyme comes from an E. coli strain that carries the cloned BamHI modification gene from Bacillus amyloliquefaciens H (ATCC 49763).

 

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Catalog # Concentration Size List Price Your Price Quantity
M0223S 4,000 units/ml 100 units $85.00
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  •  

    Product Source

    An E. coli strain that carries the cloned BamHI modification gene from Bacillus amyloliquefaciens H (ATCC 49763).
    This product is related to the following categories:
    Methyltransferases for Epigenetics Products,
    Methyltransferases Products

    • Reagents Supplied

      The following reagents are supplied with this product:

      NEB # Component Name Component # Stored at (°C) Amount Concentration

    • Properties & Usage

      Unit Definition

      One unit is defined as the amount of enzyme required to protect 1 µg λ DNA in 1 hour at 37°C in a total reaction volume of 10 µl against cleavage by BamHI restriction endonuclease.

      Reaction Conditions

      1X BamHI Methyltransferase Reaction Buffer
      Supplement with 80 µM S-adenosylmethionine (SAM)
      Incubate at 37°C

      Control Protection Assay

      BamHI Methyltransferase is incubated with 1 µg λ DNA in 10 µl 1X BamHI Methyltransferase Buffer, supplemented with 80 µM S-adenosylmethionine, for one hour at 37°C followed by 15 minutes at 65°C. The extent of protection by BamHI Methyltransferase is determined by the addition of 40 µl NEBuffer 1 supplemented with 10 mM MgCl2 and 10 units of BamHI restriction endonuclease. Incubation at 37°C for 30 minutes is followed by analysis on agarose gels.

      Storage Buffer

      50 mM Tris-HCl
      1 mM DTT
      10 mM EDTA
      200 µg/ml BSA
      50% Glycerol
      pH 7.5 @ 25°C

      Heat Inactivation

      No

    • Related Products

      Materials Sold Separately

    • Product Notes

      1. Storage of SAM: S-adenosylmethionine is stored at –20°C as 32 mM solutiondissolved in sulfuric acid (0.005 M) and 10% ethanol. SAM in this solutionstored under ideal conditions remains active for up to 6 months. SAM is unstableat (pH 7.5), 37°C, and should be replenished for reactions incubated longer than4 hours. Many problems in achieving complete digestion can be alleviated byusing fresh SAM.

    • References

      1. Hoffman, J.L. (1986). Biochemistry. 25, 4444-4449.

  • Protocols, Manuals & Usage

  • FAQs & Troubleshooting

  • Citations & Technical Literature

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