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NEBNext® Multiplex Small RNA Library Prep Set for SOLiD™ (Set 1)

This product has been discontinued. NEBNext® Small RNA Library Prep Set for SOLiD™ (E6160S/L) is a suitable alternative for most applications.

  • Catalog # E7450 was discontinued on July 11, 2014
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  • The NEBNext Multiplex Small RNA Library Prep Set for SOLiD (Set 1) contains adaptors, primers, enzymes and buffers that are ideal to convert small RNA into cDNA libraries for next-generation sequencing on the SOLiD platform (Life Technologies, Inc.). Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.

    Small RNA Library Prep Workflow
    Novel Protocol that Results in Higher Yields and Lower Adaptor-dimer Contamination (Patent Pending):
    E7450
    Functional Validation
    Each set of reagents is functionally validated together through construction and sequencing of an RNA library on the SOLiD™ 4.

    GenomeWeb article reviews NEBNext Small RNA Kits.

    Lot Control

    The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
    This product is related to the following categories:
    Next Generation Sequencing Library Preparation

    • Properties & Usage

      Materials Required but not Supplied

      • 3M Sodium Acetate. pH 5.2
      • 100% Ethanol
      • 80% Ethanol 
      • Corning®, Costar®, Spin-X® Centrifuge Tube Filters (Cellulose Acetate Filters)(Sigma Aldrich, #CLS8162)
      • RNase-free Disposable Pellet Pestles® (Kimble Kontes Asset Management, Inc., #749521-1590) 
      • 6% Novex® TBE PAGE gel, 1.0 mm, 10 well (Life Technologies, Inc., E-C6265BOX) 
      • SYBR® Gold Nucleic Acid Gel Stain (Life Technologies, Inc., S-11494)

    • Product Notes

      1. Kit Optimization:
        The components of this kit were collectively optimized with FirstChoice® Human Brain Reference RNA (Life Technologies, Inc. #AM6050).

    • Citations

      NEBNext Product Citations Search Tool

      Use this tool to find citations related to NEBNext products. Search by product, keyword or instrument name – use the filters to select specific product areas.

  • Quality, Safety & Legal

    • Quality Control Assays

      Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

    • Legal and Disclaimers

      Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

      New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

      Licenses

      Notice to Purchaser: Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. (See Patent Nos. 5,475,096; 5,670,637; 5,696,249; 5,874,557; and 5,693,502). New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the aptamer-based OneTaq® Hot Start 2X Master Mix with Standard Buffer for RESEARCH PURPOSES ONLY. Commercial use of the aptamer-based OneTaq® Hot Start 2X Master Mix with Standard Buffer requires a license from New England Biolabs, Inc. Please contact busdev@neb.com for more information.

      Trademarks



      NEBNEXT®, ONETAQ®, PROTOSCRIPT® and QUICK-LOAD® are registered trademarks of New England Biolabs, Inc.

      BIOANALYZER® is a registered trademark of Agilent Technologies, Inc.
      CORNING®, COSTAR® & SPIN-X® are registered trademarks of Corning, Inc.
      IGEPAL® is a registered trademark of Rhodia Operations.
      FIRSTCHOICE® , NOVEX® & SYBR® are registered trademarks of Life Technologies, Inc.
      PELLET PESTLE® is a registered trademark of Kimble Kontes Asset Management, Inc.
      QIAQUICK® is a registered trademark of Qiagen, Inc.
      SOLID™ is a trademark of Life Technologies, Inc.
      FICOLL™ is a trademark of GE Healthcare.

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