NEBNext^® rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
E6350S     Multi-temperature       NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) E6310S -20       RNase H Reaction Buffer E6312-2VIAL -20 1 x 0.012 ml Not Applicable   NEBNext rRNA Depletion Solution E6313-2VIAL -20 1 x 0.01 ml Not Applicable   NEBNext Probe Hybridization Buffer E6314-2VIAL -20 1 x 0.012 ml Not Applicable   DNase I Reaction Buffer E6315-2VIAL -20 1 x 0.03 ml Not Applicable   DNase I (RNase-free) E6316-2VIAL -20 1 x 0.015 ml Not Applicable   Nuclease-free Water E6317-2VIAL -20 1 x 0.4 ml Not Applicable   NEBNext RNase H E6318-2VIAL -20 1 x 0.012 ml Not Applicable   NEBNext® RNA Sample Purification Beads E6351S 4       NEBNext® RNA Sample Purification Beads E6351SVIAL 4 1 x 0.66 ml Not Applicable
E6350L     Multi-temperature       NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) E6310L -20       RNase H Reaction Buffer E6312-3VIAL -20 1 x 0.048 ml Not Applicable   NEBNext rRNA Depletion Solution E6313-3VIAL -20 1 x 0.024 ml Not Applicable   NEBNext Probe Hybridization Buffer E6314-3VIAL -20 1 x 0.048 ml Not Applicable   DNase I Reaction Buffer E6315-3VIAL -20 1 x 0.12 ml Not Applicable   DNase I (RNase-free) E6316-3VIAL -20 1 x 0.06 ml Not Applicable   Nuclease-free Water E6317-3VIAL -20 1 x 1.5 ml Not Applicable   NEBNext RNase H E6318-3VIAL -20 1 x 0.048 ml Not Applicable   NEBNext® RNA Sample Purification Beads E6351L 4       NEBNext® RNA Sample Purification Beads E6351LVIAL 4 1 x 2.64 ml Not Applicable
E6350X     Multi-temperature       NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) E6310X -20       RNase H Reaction Buffer E6312-4VIAL -20 1 x 0.192 ml Not Applicable   NEBNext rRNA Depletion Solution E6313-4VIAL -20 1 x 0.096 ml Not Applicable   NEBNext Probe Hybridization Buffer E6314-4VIAL -20 1 x 0.192 ml Not Applicable   DNase I Reaction Buffer E6315-4VIAL -20 1 x 0.48 ml Not Applicable   DNase I (RNase-free) E6316-4VIAL -20 1 x 0.24 ml Not Applicable   Nuclease-free Water E6317-4VIAL -20 1 x 6 ml Not Applicable   NEBNext RNase H E6318-4VIAL -20 1 x 0.192 ml Not Applicable   NEBNext® RNA Sample Purification Beads E6351X 4       NEBNext® RNA Sample Purification Beads E6351XVIAL 4 1 x 10.6 ml Not Applicable

1. Adiconis, X. et al (2013). Comparative analysis of RNA sequencing methods for degraded or low-input samples. Nature Methods. 10, 623-629.
2. Morlan, J.D. et al. (2012). Selective depletion of RNA enables whole transcriptome profiling of archival fixed tissue. PLos One. 77, e42882.

1. Protocol for use with NEBNext^® rRNA Depletion Kit (Human/Mouse/Rat) (E6310, E6350)

1. Can I use this product with degraded RNA or fragmented RNA?
2. What total RNA input should I use?
3. What is the percentage of rRNA left after depletion?
4. What is the percentage of rRNA left after depletion?
5. I am currently using RNAClean XP magnetic beads. Will the NEBNext RNA Sample Purification Beads be a suitable replacement?
6. To remove ribosomal RNA from total RNA, should I use the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) or the NEBNext rRNA Depletion Kit (NEB #E6310)?
7. Does the NEBNext^® rRNA Depletion Kit (Human/Mouse/Rat) work for ribosomal footprinting or profiling application?
8. Does the NEBNext^® Globin mRNA & rRNA Depletion Kit (Human/Mouse/Rat) work for ribosomal footprinting or profiling applications?
9. Can the enriched RNA resulting from the NEBNext depletion kit be used in long read sequencing applications?

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Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• E6350S_L_X_v1

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• RNase H Reaction Buffer
• NEBNext rRNA Depletion Solution
• NEBNext Probe Hybridization Buffer
• DNase I Reaction Buffer
• DNase I (RNase-free)
• Nuclease-free Water
• NEBNext RNase H
• NEBNext^® RNA Sample Purification Beads

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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