FAQ: To remove ribosomal RNA from total RNA, should I use the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) or the NEBNext rRNA Depletion Kit (NEB #E6310)?
The NEBNext rRNA Depletion Kit can be used with low quality Total RNA samples. The NEBNext Poly(A) mRNA Magnetic Isolation Module requires high quality Total RNA.