The NEBNext dA-Tailing Module enables incorporation of a non-templated dAMP on the 3´ end of a blunt-ended DNA fragment. The module is optimized for use with the NEBNext Quick Ligation Module (NEB #E6056), and is part of the original standard DNA library prep workflow for Illumina sequencing, which is suitable for 1–5 µg of input DNA.
Each kit component must pass rigorous quality control standards, and for each new lot the entire set of reagents is functionally validated together by construction and sequencing of an indexed library on the Illumina sequencing platform.
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