The NEBNext UltraExpress™ FS DNA Library Prep Kit is the latest generation of NEBNext DNA library prep, with a fast, streamlined workflow, including enzymatic fragmentation, end prep, and dA-tailing with a single enzyme mix. In under 2 hours, the protocol enables the creation of high yield, high quality libraries, from a broad input amount range, while generating less plastic waste. Designed for simplicity and efficiency, the kit features a single protocol for all input amounts, making NEBNext UltraExpress FS DNA Library Prep well suited for all throughputs.
Fast workflow (< 2 hours), including FS "Fragmentation System" enzymatic fragmentation
Fewer steps, requiring only a single reaction tube
Fewer clean-up steps required
High-quality libraries from a wide input range (10 - 200 ng intact DNA)
The NEBNext UltraExpress FS DNA Library Prep Kit has been developed in response to user need for a faster, streamlined DNA prep workflow. This kit integrates enzymatic fragmentation and delivers high quality libraries from a variety of sample types. It features a single protocol for all DNA inputs, ranging from 10 – 200 ng of intact DNA. The workflow incorporates master mixed reagents, reduced incubation times, and fewer cleanup steps. Use of this kit also generates less plastic consumable waste because the entire library prep is conducted in a single tube.
Go from intact sample to library in under 2 hours with FS (Fragmentation System) enzymatic fragmentation
Single-protocol simplicity cuts down on reaction setup time, while streamlined workflows speed up library prep
A single-tube workflow means less plastic and consumable waste
Flexibility is enabled with simple guidelines for customized protocols, if desired
Automation-friendly protocols for enhanced scalability
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