The NEBNext UltraExpress Library Prep kits are compatible with both the NEBNext truncated, non-indexed adaptor, supplied with the NEBNext index primer sets and the full-length unique dual index (UDI) unique molecular identifier (UMI) adaptors. For a complete list of all compatible oligos, please see the NEBNext Multiplex Oligos Selection Chart.
To use the full-length Unique Dual Index UMI adaptors (e.g., NEB #E7395), optimization of adaptor dilution and cleanup conditions may be required.