Home Protocols Where can I find protocols for using the NEBNext UltraExpress® FS DNA Library Prep Kit NEB #E3340)?

Where can I find protocols for using the NEBNext UltraExpress® FS DNA Library Prep Kit NEB #E3340)?

Protocols for using NEBNext UltraExpress® FS DNA Library Prep Kit (NEB #E3340) reagents can be found within the product manual, including:

  • Fragmentation/End Prep
  • Adaptor Ligation
  • PCR enrichment of Adaptor-ligated DNA
  • Phased Bead Cleanup of PCR Reaction
  • Appendix A: Recommendations for customized fragmentation time and/or cleanup to alter library size distributions
  • Appendix B: Recommendations for customized cycling per input