GMP-grade Products for RNA Synthesis – Tools to Take you from Template to Transcript

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For almost 50 years, NEB® has been a world leader in the discovery and production of reagents for the life science industry. Our enzymology expertise effectively positions us to supply reagents for the synthesis of high-quality RNA – from template generation and transcription, to capping, tailing and cleanup after synthesis. These products are designed and manufactured based on decades of molecular biology experience, so that you can be confident they will work for your application.


From research to therapeutic production, NEB’s in vitro transcription portfolio will meet your needs

NEB’s portfolio of research-grade and GMP-grade* products enables bench-scale to commercial-scale mRNA manufacturing. Our optimized HiScribe® kits enable convenient in vitro transcription (IVT) workflows. When it is time to scale up and optimize reaction components, our standalone reagents are readily available in formats matching our GMP-grade offering, enabling a seamless transition to large-scale therapeutic mRNA manufacturing.


Enabling gram-scale RNA synthesis

NEB manufactures and inventories the following enzyme specificities at GMP-grade, meeting customer needs with short lead times:

  • Vaccinia Capping Enzyme (NEB #M0280) – A full system for enzymatic capping based on the Vaccinia virus Capping Enzyme (VCE)

  • Faustovirus Capping Enzyme (NEB #M0281) – Improved capping efficiency and broad temperature range compared to Vaccinia Capping enzyme (Coming soon)

  • T7 RNA Polymerase (NEB #M0251) – RNA Polymerase used for in vitro mRNA synthesis, and is highly specific for the T7 phage promoter

  • mRNA Cap 2′-O-Methyltransferase (NEB #M0366) – mRNA Cap 2´-O-Methyltransferase adds a methyl group at the 2′-O position of the first nucleotide adjacent to the cap structure at the 5´ end of the RNA

  • RNase Inhibitor, Murine (NEB #M0314) – RNase Inhibitor, Murine, specifically inhibits RNases A, B and C

  • Pyrophosphatase, Inorganic (E. coli) (NEB #M0361) – Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate

  • DNase I (RNase-free) (NEB #M0303) – DNA-specific endonuclease used for removal of contaminating genomic DNA from RNA samples and degradation of DNA templates in transcription reactions

  • HiScribe T7 High Yield RNA Synthesis Components (NEB #E2040) – Separate components available in GMP-grade format

  • BspQI (NEB #R0712) – Type IIS restriction enzyme and Isoschizomer of LguI and SapI used to linearize plasmid DNA for mRNA therapeutics

  • phi29 DNA Polymerase (NEB #M0269) – has exceptional strand displacement and processive synthesis properties and an inherent 3´→5' proofreading exonuclease activity

  • TelN Protelomerase (NEB #M0651) – Cuts dsDNA at a TelN recognition sequence (56 bp) and leaves covalently closed ends at the site of cleavage

Not sure whether you need research-grade or GMP-grade products?
Learn more about the difference between GMP-grade and research-grade products

Template Generation TableCaret_GMPgrade In vitro Transcription TableCaret_GMPgrade RNA Capping TableCaret_GMPgrade Poly(A) Tailing TableCaret_GMPgrade RNAPurification
Q5® High-Fidelity DNA Polymerase HiScribe T7 mRNA Kit with CleanCap® Reagent AG E. coli Poly(A) Polymerase Monarch® RNA Cleanup Kit (10 μg)
HiScribe™ T7 ARCA mRNA Synthesis Kit (with tailing)

phi29 DNA Polymerase

 GMPgrade_icon HiScribe T7 ARCA mRNA Synthesis Kit Monarch RNA Cleanup Kit (50 μg)

TelN Protelomerase

 GMPgrade_icon HiScribe T7 High Yield RNA Synthesis Components Faustovirus Capping Enzyme GMP_ComingSoon_Icon_42w   Monarch RNA Cleanup Kit (500 μg)
Vaccinia Capping System GMPgrade_icon
dNTP solution mixes HiScribe T7 Quick High Yield RNA Synthesis Kit   mRNA Cap 2′-O-Methyltranferase GMPgrade_icon   Lithium Chloride
BspQI*   GMPgrade_icon HiScribe SP6 High Yield RNA Synthesis Kit   ARCA and other mRNA cap analogs      

DNA Assembly:

 T3 & SP6 RNA Polymerases          
  T7 RNA Polymerase GMPgrade_icon        
 

Hi-T7 RNA Polymerase

          
  Companion Products         Companion Products
  RNase Inhibitor (Murine) GMPgrade_icon       Monarch RNA Cleanup Binding Buffer
  RNase Inhibitor (Human Placental)         Monarch RNA Cleanup Wash Buffer
  Pyrophosphatase, Inorganic (E. coli) GMPgrade_icon       Nuclease-free Water
  Pyrophosphatase, Inorganic (Yeast)          
  DNase I (RNase-free) GMPgrade_icon        
  DNase I-XT
          
  NTPs          

GMPgrade_icon = available in GMP-grade

Fluorescent_GMPgradeComparison
Functional expression of active green fluorescent protein in HEK 293
cells after transfection of capped poly-adenylated mRNA demonstrates
qualitative equivalence of GMP-grade and research-grade reagents.

 
* "GMP-grade" is a branding term NEB uses to describe products manufactured or finished at NEB’s Rowley facility. The Rowley facility was designed to manufacture products under more rigorous infrastructure and process controls to achieve more stringent product specifications and customer requirements. Products manufactured at NEB’s Rowley facility are manufactured in compliance with ISO 9001 and ISO 13485 quality management system standards. However, at this time, NEB does not manufacture or sell products known as Active Pharmaceutical Ingredients (APIs), nor does NEB manufacture its products in compliance with all of the Current Good Manufacturing Practice regulations.

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. See www.neb.com/trademarks. The use of products may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

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