The Monarch Spin DNA Gel Extraction Kit offers an efficient method for DNA gel extraction and purification, enabling up to 5 μg of high-quality DNA. This new version features upgraded spin columns precision-engineered to uniquely allow for low elution, in as little as 5 μl, for highly concentrated DNA in downstream applications such as sequencing, restriction digestion, library construction, labeling and other enzymatic manipulations. The kit includes spin columns, collection tubes, buffers, and other reagents in compact and streamlined packaging for silica membrane-based purification and DNA gel extraction. Our optimized gel dissolving buffer allows efficient gel dissolving and DNA binding on the column, without a need to monitor pH or add isopropanol. These kits contain uniquely designed columns and bottles to significantly reduce plastic usage compared to leading suppliers, while maintaining structural integrity for centrifugation, vacuum manifolds and other steps. The kit features Monarch spin columns that are manufactured using state of the art automation machinery for high quality and consistency.
Properties
Purification format
Spin column for nucleic acid purification
Compatible methods
Centrifugation or vacuum manifold
DNA sample
dsDNA from agarose gels
Typical recovery
70-90% for < 10 kb and 50-70% for ≥ 10 kb
DNA Purity
A260/280 > 1.8 and A260/230 > 1.8
Binding Capacity
Up to 5 μg
Elution Volume
≥ 5 μl
DNA Size Range
Standard protocol: 100 bp – 25 kb
Protocol Time
10 - 15 minutes of spin and incubation time
Compatible Downstream Applications
Ligation, restriction digestion, labeling and other enzymatic manipulations, library construction and DNA sequencing
Figure 1: The Monarch Spin DNA Gel Extraction Kit consistently recovers DNA as well as or better than the leading supplier, and is more concentrated for greater downstream utility
Using the Monarch Spin DNA Gel Extraction Kit, DNA concentrations and recovery are equal to or higher than a leading suppliers' kits. 2 μg of 3 kb fragment were resolved on a 1.2 % w/v TBE agarose gel, excised and processed with recommended protocols and elution volumes. Concentrations of DNA were measured using a Trinean DropSense 16 and percent recovery calculations are based on the eluted DNA concentration and elution volume used.
Figure 2: DNA purified from agarose gels using Monarch Spin DNA Gel Extraction Kit yields high-quality DNA, suitable for downstream applications
Use of the Monarch Spin DNA Gel Extraction Kit can effectively purify DNA from agarose and is suitable for downstream applications, performing as well as a leading supplier’s kits. 2 μg of 3 kb fragments were resolved on a 1.2 % w/v TBE agarose gel, excised, and processed with recommended protocols. 1 μg of DNA was ligated using the Blunt/TA Ligase Master Mix (NEB #M0367). The equivalent amount of eluate and the ligated samples were resolved on a 1.2 % TBE agarose gel. M = 1 kb DNA Ladder (NEB #N3232).
Figure 3: Monarch Spin DNA Gel Extraction Kit is effective for a wide range of DNA sizes
A mixture of 7 DNA fragments (lane I) ranging from 0.5 kb to 10 kb was prepared and resolved on a 1.2 % w/v TBE agarose gel. Each fragment was manually excised from the agarose gel and processed using the Monarch® Spin DNA Gel Extraction Kit. The elution of each fragment was resolved on a new gel with the original mixture for comparison.
Figure 4: Features of the Monarch Spin Column S1A
NEB® Monarch’s unique column design and membrane assembly allows high-quality DNA purification with low-elution volume, for highly-concentrated DNA for downstream applications. The column is designed and made with significantly less plastic for reduced environmental impact.
Figure 5: Overview of the Monarch Spin DNA Gel Extraction Kit workflow
The Monarch Spin DNA Gel Extraction Kit uses a dissolve/bind/wash/elute method using a spin column.
This product is related to the following categories:
High Performance: Achieve high yields (up to 5 μg) and high purity in the extraction and purification of DNA from agarose gels, with the capability to remove contaminants and residual salts.
High Concentration: Elute in very small volumes, in as little as 5 μl for elution, allowing for highly concentrated DNA.
Time Savings: A short protocol of only 10 minutes of spin and incubation time is needed to complete the workflow.
Unique Design: Spin column features a unique design that enables elution in low volumes and minimizes buffer retention and contaminant carryover.
Optimized: Buffer system is optimized, without the need to adjust pH or add isopropanol.
Application Compatibility: Purified DNA is ready for downstream molecular applications, such as restriction digests, DNA sequencing, ligation, amplification, and other enzymatic reactions.
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
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