SNAP-Cell® 647-SiR is a far-red fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells, on cell surfaces, or in vitro.
It is a cell-permeable substrate (also termed SiR-SNAP) based on 6-carboxy-tetramethylsiliconrhodamine
It is suitable for standard Cy5 filter sets
It has an excitation maximum at 645 nm and an emission maximum at 661 nm
This package contains 30 nmol
of SNAP-Cell 647-SiR substrate, sufficient to make
10 ml of a 3 μM SNAP-tag fusion protein labeling
solution.
The SNAP-tag protein labeling system enables the
specific, covalent attachment of virtually any molecule
to a protein of interest. The SNAP-tag is based on
human O6-alkylguanine-DNA-alkyltransferase (hAGT).
SNAP-tag substrates are fluorophores, biotin or beads
conjugated to guanine or chloropyrimidine leaving
groups via a benzyl linker. In the labeling reaction, the
substituted benzyl group of the substrate is covalently
attached to the SNAP-tag.
There are two steps to using this system: subcloning
and expression of the protein of interest as a
SNAP-tag fusion, and labeling of the fusion with the
SNAP-tag substrate of choice. Expression of SNAP-tag
fusion proteins is described in the documentation
supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is
described below.
Figure 1. Live NIH/3T3 cells expressing SNAP-tag fused to mitochondrial oxidase 8A (Cox8A-SNAPf)
Cells
were labeled with SNAP-Cell 647-SiR (red) for 30
minutes at 37°C, 5% CO2.Figure 2: Excitation (dotted line) and emission spectra of SNAP-Cell 647-SiR after coupling to SNAP-tag in buffer at pH 7.5 Figure 3: Structure of SNAP-Cell 647-SiR (MW 724.9 g/mol)
This product is related to the following categories:
Storage:SNAP-Cell 647-SiR should be stored at -20°C(long term) or at 4°C in the dark (short term, lessthan 4 weeks). Protect the substrate from lightand moisture. With proper storage at -20°C thesubstrate should be stable for at least three yearsdry or 3 months dissolved in DMSO.
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Licenses
Cellular Imaging and Analysis (i.e., SNAP and CLIP products)
Notice to Buyer/User: The Buyer/User has a non-exclusive license to use this system or any component thereof for RESEARCH AND DEVELOPMENT ONLY. Commercial use of this system or any components thereof requires a license from New England Biolabs, Inc., 240 County Road, Ipswich, MA 01938. For detailed information see our Terms of Use . Methods for Using O6-Alkylguanine-DNA-Alkyltransferases: US 7,939,284; US 8,367,361; EP 1 410 023; EP 2 037 271; EP 1 696 234; EP 2 211 177; JP 4195815; JP 4226053; CN 1295510; CN 1975422; and SG 100125.
Substrates for O6-Alkylguanine-DNA Alkyltransferase: US 7,799,524; and JP 4976651.
Mutants of O6-Alkylguanine-DNA-Alkyltransferases: US 7,888,090. EP 1720981 (pending).
Specific Substrates for O6-Alkylguanine-DNA-Alkyltransferases: US 8,163,479. EP 1730298 (pending).
Cell Permeable, Fluorescent Dye: US 20140349282 (pending), EP 2748173 (pending).
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