Magnetic Beads and Racks

Separation Racks

NEB Magnetic Separation Racks are designed to be used for small-scale separations using magnetic particles. Strong neodymium rare earth magnets permit rapid separation of magnetic particles from suspension. Multiple separations can be done using standard sized microcentrifuge tubes with the 6- and 12-Tube Magnetic Separation Racks (NEB #S1506, NEB #S1509), and 50 ml tubes with the 50 ml Magnetic Separation Rack (NEB #S1507). High throughput separations can be done using the 96-Well Microtiter Plate Magnetic Separation Rack (NEB #S1511) and the NEBNext^® Magnetic Separation Rack (NEB #S1515).

Magnetic Protein Purification

Chitin Magnetic Beads (NEB #E8036): An affinity matrix for small-scale isolation of target proteins fused to a chitin binding domain (CBD). Chitin beads are prepared with a magnetite core and can be regenerated without loss of binding capacity. Immobilized fusion proteins can be used in subsequent experiments to capture target proteins from crude cell lysates that interact with the immobilized fusion protein.

Amylose Magnetic Beads (NEB #E8035): An affinity matrix for small-scale isolation and purification of maltose binding protein (MBP) fusion proteins from cell culture supernatants. Amylose is covalently coupled to a paramagnetic particle through a linkage that is stable and leak resistant over a wide pH range. Immobilized fusion proteins can be used in subsequent experiments to capture target proteins from crude cell lysates that may interact with immobilized fusion protein.

Anti-MBP Magnetic Beads (NEB #E8037): An affinity matrix for small-scale isolation and purification of maltose binding protein (MBP) fusion proteins from cell culture supernatants. Monoclonal anti-MBP is covalently coupled to a nonporous paramagnetic particle through a linkage that is stable and leak resistant over a wide pH range.

Ni-NTA Magnetic Beads (NEB #S1423): an affinity matrix for the small-scale isolation and purification of His-tagged fusion proteins in manual or automated formats. They are prepared with agarose-based, super-paramagnetic microparticles that provide high binding capacity and fast magnetic response.

Protein A Magnetic Beads (NEB #S1425): an affinity matrix for the small-scale isolation and purification of immunoglobulins. Protein A exhibits high affinity for subclasses of IgG from many species including human, rabbit and mouse. The beads consist of a truncated form of recombinant Protein A that is covalently coupled to a nonporous superparamagnetic particle.

Protein G Magnetic Beads (NEB #S1430): an affinity matrix for the small-scale isolation and purification of immunoglobulins. Protein G exhibits high affinity for subclasses of IgG from many species including human, rabbit, mouse, rat and sheep. The beads consist of a truncated form of recombinant Protein G that is covalently coupled to a nonporous superparamagnetic particle.