This product was discontinued on December 15, 2022 and is replaced with Ph.D.-12 Phage Display Peptide Library Kit v2 (NEB #E8210).
The Ph.D.-12™ Phage Display Peptide Library Kit contains a tube of the Ph.D.-12 Phage Library, protein reagents for a control reaction and sequencing primer stocks. The Ph.D.-12 library is a combinatorial library of random 12-mer peptides fused to a minor coat protein (pIII) of M13 phage. The displayed peptide is expressed at the N-terminus of pIII. The library consists of approximately 109 electroporated (i.e., unique) sequences.
The Ph.D.-12 Phage Display Peptide Library is based on a combinatorial library of random dodecapeptides fused to a minor coat protein (pIII) of M13 phage (1–6). The displayed peptide (12-mer) is expressed at the N-terminus of pIII, i.e., the first residue of the mature protein is the first randomized position. The peptide is followed by a short spacer (Gly-Gly-Gly-Ser) and then the wild-type pIII sequence. The library consists of approximately 109 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage.
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