Luna® Universal qPCR & RT-qPCR

Lighting the way – Luna® Universal qPCR & RT-qPCR banner with luna moth

Luna products deliver sensitive, reliable qPCR and RT-qPCR performance across a wide range of targets, sample types and workflows. A blue visible dye assists in tracking the reagents when pipetting into clear PCR plates or tubes. 

The following Luna qPCR products contain a universal passive reference dye that enables compatibility with a variety of real-time instruments, including those that require No ROX, Low ROX, or High ROX passive reference dye concentrations:

Luna Universal qPCR Master Mix (NEB #M3003)
Luna Universal Probe qPCR Master Mix (NEB #M3004)
Luna Universal One-Step RT-qPCR Kit (NEB #E3005)
Luna Universal Probe One-Step RT-qPCR Kit (NEB #E3006)
Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB #M3019)
LyoPrime Luna Probe One-Step RT-qPCR Mix with UDG (NEB #L4001)
Luna Direct Probe One-Step RT-qPCR 4X Mix with UDG (NEB #M3052)
Luna Direct Probe qPCR 4X Master Mix with UDG (NEB #M3055) 

For instruments that do not utilize ROX normalization, the following products contains no reference dye:
Luna Probe One-Step RT-qPCR Kit (No ROX) (NEB #E3007)
Luna Probe One-Step RT-qPCR 4X Mix with UDG (No ROX) (NEB #M3029)
Luna Direct Probe One-Step RT-qPCR 4X Mix with UDG (No ROX) (NEB #M3053)
Luna Direct Probe qPCR 4X Master Mix with UDG (No ROX) (NEB #M3056)  

The Luna Direct Probe One-Step RT-qPCR (NEB #M3052) and qPCR (NEB #M3055) 4X Mixes with UDG enable reliable amplification directly from crude or minimally processed samples. Inhibitor-tolerant formulations and higher sample input capacity increase assay sensitivity, help simplify workflows, and accelerate results.

The Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB #M3019) supports robust, sensitive detection and quantitation of up to 5 targets in a multiplexed reaction. It is supplied at a 4X concentration and enables higher amounts of sample input.

The LyoPrime Luna Probe One-Step RT-qPCR Mix with UDG (NEB #L4001) offers the same versatile features and strong performance as the M3019 product in a lyophilized format. For more information on lyophilized products used for RNA detection, please visit www.neb.com/lyoprime.

The Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit (NEB #E3019) combines the M3019 product with an optimized SARS-CoV-2 Primer/Probe Mix specific to two regions of the SARS-CoV-2 virus N gene [based on sequences provided by the Centers for Disease Control and Prevention (CDC)].

The other Luna products contain dUTP and enable carryover prevention when reactions are treated with Antarctic Thermolabile UDG (NEB #M0372). A blue visible dye assists in tracking the reagents when pipetting into clear, multi-welled PCR plates. The reverse transcriptase featured in the Luna One-Step RT-qPCR products is an engineered WarmStart® enzyme developed for robust performance and increased thermostability. These features, combined with rapid, sensitive and precise real-time qPCR performance, make Luna the universal choice for all your qPCR and RT-qPCR experiments.

For two-step RT-qPCR, the LunaScript® RT SuperMix Kit (NEB #E3010) offers a fast (less than 15 min), robust, and sensitive option for cDNA synthesis upstream of your Luna qPCR experiment.

The LunaScript RT Master Mix Kit (Primer-free) (NEB #E3025) features an optimized 5X master mix containing all the necessary components for first strand cDNA synthesis, except primers. The mix is compatible with random primers, oligo dT primers, and gene-specific primers, enabling maximum cDNA synthesis flexibility for 2-step RT-qPCR, 2-step RT-PCR, and RNA-seq applications.

The Luna Cell Ready Lysis Module (NEB #E3032) and kits are designed for direct RNA quantitation from cell lysate, bypassing traditional RNA extraction and purification steps. Coordinated cell lysis, RNA release, and genomic DNA removal is achieved in a 15 min protocol, with optimal RT-qPCR analysis obtained when the cell lysate is paired with Luna Universal One-Step RT-qPCR Kits.

Make a simpler choice 

  • Convenient master mix and supermix formats with user-friendly protocols simplify reaction setup
  • Non-interfering, visible tracking dye helps to eliminate pipetting errors
  • Read our white paper on key considerations for optimal lyophilized reagent development
Learn more

Experience best-in-class performance

  • All Luna products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
  • Products perform consistently across a wide variety of sample sources
  • A comprehensive evaluation of commercially-available qPCR and RT-qPCR reagents demonstrates superior performance of Luna products

Learn more

Optimize your One-Step RT-qPCR with Luna WarmStart® Reverse Transcriptase 

  • Novel, thermostable reverse transcriptase (RT) improves performance
  • WarmStart RT paired with Hot Start Taq. Unique aptamer technology increases reaction specificity and robustness.
  • Skip RNA purification and go direct from cells to RT-qPCR analysis with Luna Cell Ready Module and kits

Learn more

NEBs Luna products are optimized for qPCR or RT-qPCR and are available for either intercalating dye or probe-based detection methods. The Luna Cell Ready Lysis Module allows for streamlined, direct RNA quantitation.Interested in learning more about
Luna qPCR & RT-qPCR products?
Download our product brochure

Available products:

Luna Universal qPCR Master Mix

Dye-based qPCR for DNA and cDNA
Luna Universal Probe qPCR Master Mix

Probe-based qPCR for DNA and cDNA
Luna Universal One-Step RT-qPCR Kit

Dye-based one-step RT-qPCR for RNA
Luna Universal Probe One-Step RT-qPCR Kit

Probe-based one-step RT-qPCR for RNA
Luna Probe One-Step RT-qPCR 4X Mix with UDG

4X mix for probe-based one-step RT-qPCR
LyoPrime Luna Probe One-Step RT-qPCR Mix with UDG

Single tube with blue tracking dye includes everything needed for first strand cDNA synthesis in two-step RT-qPCR workflows

Luna Direct Probe One-Step RT-qPCR 4X Mix with UDG

Supports direct probe-based one-step RT-qPCR from crude or purified RNA samples 

Luna Direct Probe qPCR 4X Master Mix with UDG

Supports direct probe-based qPCR from crude or purified DNA samples 
Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit

Flexible first-strand cDNA synthesis with user-selected primers
LunaScript RT SuperMix Kit

Single tube with blue tracking dye includes everything needed for first strand cDNA synthesis in two-step RT-qPCR workflows
LunaScript RT Master Mix Kit (Primer-free)

Flexible first-strand cDNA synthesis with user-selected primers
Luna Cell Ready One-Step RT-qPCR Kit

Cell lysis module + dye-based RT-qPCR kit for direct detection from cell lysates
Luna Cell Ready Probe One-Step RT-qPCR Kit

Cell lysis module + probe-based RT-qPCR kit for direct detection from cell lysates
Luna Cell Ready Lysis Module

Cell lysis module alone

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Videos

  • Overview of qPCR

    Learn the basics of qPCR in this short animation.                                                                                                  

  • NEB® TV Ep. 16 – Overview and Applications of qPCR

    Learn the basics of qPCR, and hear more about our Luna products, including our novel method for data analysis.

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.