NEBridge® Golden Gate Assembly

Choose NEBridge for
Golden Gate Assembly

In need of efficient, seamless, single or multiple fragment DNA assembly in a single reaction? Our portfolio of NEBridge products can support your assembly needs.

• Precise, scarless DNA assembly of 2–50+ fragments in a single reaction, using Type IIS restriction enzymes
• Efficient with repetitive and high GC content sequences
• Compatible with a broad range of fragment sizes (<100 bp to >15 kb)

Drawing of a tube




NEBridge Ligase Master Mix

Try our convenient 3X Master Mix for use with your choice of NEB Type IIS restriction enzyme, for efficient and accurate Golden Gate Assembly.

NEBridge Golden Gate Assembly

Use this tool to design primers for your Golden Gate DNA Assembly, predict overhang fidelity, or find optimal Golden Gate junctions for assembling long sequences.

Tool Try Our Tool
Golden Gate Assembly domestication tutorial

This video discusses domestication, or the removal of Type IIS cutsites naturally occurring in vector or insert sequences, as it relates to Golden Gate Assembly.

View Tutorial
Technical tips for optimizing Golden Gate Assembly reactions

Looking to assemble multiple DNA fragments in a single reaction? Here are some tips to keep in mind when planning your Golden Gate Assemblies.

Find Technical Tips

Featured NEBridge Golden Gate Assembly products

NEBridge Golden Gate Assembly is used in synthetic biology to efficiently and seamlessly assemble multiple DNA fragments for in vitro transcription, library generation, multiple site-directed mutagenesis and more.

NEB offers a wide range of Type IIS restriction enzymes, NEBridge Golden Gate Assembly kits and master mixes to help you get the results you want, faster.

This content is covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.

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