NEBridge® Golden Gate Assembly
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Golden Gate Assembly
In need of efficient, seamless, single or multiple fragment DNA assembly in a single reaction? Our portfolio of NEBridge products can support your assembly needs.
• Precise, scarless DNA assembly of 2–50+ fragments in a single reaction, using Type IIS restriction enzymes
• Efficient with repetitive and high GC content sequences
• Compatible with a broad range of fragment sizes (<100 bp to >15 kb)
NEBridge Ligase Master Mix
Try our convenient 3X Master Mix for use with your choice of NEB Type IIS restriction enzyme, for efficient and accurate Golden Gate Assembly.
NEBridge Golden Gate Assembly
Use this tool to design primers for your Golden Gate DNA Assembly, predict overhang fidelity, or find optimal Golden Gate junctions for assembling long sequences.
Tool Try Our Tool
Golden Gate Assembly domestication tutorial
This video discusses domestication, or the removal of Type IIS cutsites naturally occurring in vector or insert sequences, as it relates to Golden Gate Assembly.
View Tutorial
Technical tips for optimizing Golden Gate Assembly reactions
Looking to assemble multiple DNA fragments in a single reaction? Here are some tips to keep in mind when planning your Golden Gate Assemblies.
Find Technical TipsFeatured NEBridge Golden Gate Assembly products
NEBridge Golden Gate Assembly is used in synthetic biology to efficiently and seamlessly assemble multiple DNA fragments for in vitro transcription, library generation, multiple site-directed mutagenesis and more.
NEB offers a wide range of Type IIS restriction enzymes, NEBridge Golden Gate Assembly kits and master mixes to help you get the results you want, faster.
NEBridge Golden Gate Assembly Kit (BsmBI-v2)
NEBridge Golden Gate Assembly Kit (BsmBI-v2)
This kit allows for efficient, seamless cloning and assembly of 2–50+ DNA fragments using the Type IIS restriction enzyme BsmBI-v2 (NEB #R0739), optimized for Golden Gate Assembly.
NEBridge Golden Gate Assembly Kit (BsaI-HFv2)
NEBridge Golden Gate Assembly Kit (BsaI-HFv2)
This kit allows for efficient, seamless cloning and assembly of 2–50+ DNA fragments using the Type IIS restriction enzyme BsaI-HFv2 (NEB #R3733), optimized for Golden Gate Assembly.
NEBridge Ligase
Master Mix
NEBridge Ligase
Master Mix
This 3X master mix for Golden Gate Assembly is designed for use with NEB Type IIS restriction enzymes. It contains T4 DNA Ligase (NEB #M0202) in an optimized reaction buffer with a proprietary ligation enhancer.
PaqCl®
PaqCl®
This new Type IIS restriction enzyme has a 7-base recognition sequence, which reduces the need for domestication, or removal of internal restriction sites.
This content is covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.
