Home Forms NEBridge^® Ligase Master Mix Sample Request Form

NEBridge^® Ligase Master Mix Sample Request Form

To request a sample of NEBridge Ligase Master Mix, please complete the form below. Limit one sample per customer.

For more information about NEBridge Golden Gate Assembly please visit www.NEB.com/GoldenGate.

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To request a sample of NEBuilder HiFi DNA Assembly Mix, please complete the form below. Limit one sample per customer.

For more information about NEBuilder HiFi, The Next Generation of DNA Cloning, please visit NEBuilderHiFi.com .

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Thank you for your inquiry. We regret to inform you that under current U.S. law, NEB is prohibited from conducting business with individuals and entities in your country of residence. As such, we are unable to respond to your request at this time.

Note: Shipments to metropolitan France only.

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What Type IIS restriction enzyme are you planning on using with the NEBridge Ligase Master Mix? (Select all that apply)

BbsI-HF®	BsaI-HF®v2
BsmBI-v2	BspQI
BtgZI	Esp3I
PaqCI®	Sapl
Other

Other

How many fragments do you plan on assembling in the NEBridge Ligase Master Mix reaction? (Select all that apply if performing multiple experiments)

2 (one insert + one destination vector)3-67-1213-2425-3536+

What application(s) will you be performing with the NEBridge Ligase Master Mix? (Select all that apply)

Plasmid generation/CloningTemplate construction for in vitro transcriptionMetabolic pathway engineeringCRISPR array generationCombinatorial library generationGenome engineeringOther

Other

Have you performed Golden Gate Assembly before?

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Do you currently use or plan on using automated liquid handlers for your Golden Gate Assembly?

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Your purchase, acceptance, and/or payment of and for NEB’s products is pursuant to NEB’s Terms of Sale. NEB does not agree to and is not bound by any other terms or conditions, unless those terms and conditions have been expressly agreed to in writing by a duly authorized officer of NEB.

By requesting and accepting a free product sample, you represent that you are not prohibited by law, regulation (including Office of Govt Ethics 5 C.F.R. 2635.201-206), contract, policy, or terms of employment from doing so.

Videos

Golden Gate Assembly Workflow

Find out how Golden Gate Assembly can be used to quickly join multiple DNA fragments.
Listen to DAD (Data-optimized Assembly Design) when constructing high-complexity Golden Gate Assembly targets

This webinar introduces insights and modified protocols for Golden Gate Assembly enabling 50+ DNA fragments to be faithfully assembled in a single reaction.
Golden Gate Assembly Domestication Tutorial

This video discusses Domestication, or the removal of Type IIS cut sites naturally occurring in vector or insert sequences, as it relates to Golden Gate Assembly.
Golden Gate Assembly Tool Tutorial

This video demonstrates how to use the Golden Gate Assembly Tool, we will walk through selecting insert and plasmids, primer design to make amplicon inserts.
Restriction Enzymes in Golden Gate Assembly

Type IIS restriction enzymes have both recognition and binding sites, but cut downstream of the recognition site, creating 4-base overhangs ideal for re-assembly. View a list of TypeIIS enzymes.

Videos

Golden Gate Assembly Workflow

Listen to DAD (Data-optimized Assembly Design) when constructing high-complexity Golden Gate Assembly targets

Golden Gate Assembly Domestication Tutorial

Golden Gate Assembly Tool Tutorial

Restriction Enzymes in Golden Gate Assembly

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