FAQ: Can I use T7 Endonuclease I genome editing (CRIPSR/Cas9,TALEN,ZFN) mismatch detection assays using unpurified PCR products?
-Q5® High-Fidelity DNA Polymerase with Q5® Reaction Buffer -OneTaq® DNA Polymerase. Under these conditions, purification of PCR products before digestion with T7 Endonuclease I is optional. For purification of PCR products we recommend the Monarch PCR & DNA Cleanup kit.