Monarch^® Plasmid Lysis Buffer (B2)

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
T1012L     25       Monarch® Plasmid Lysis Buffer (B2) T1012-3 25 2 x 27 ml Not Applicable

Companion Products

• Monarch® Plasmid Miniprep Kit 
• Monarch® Plasmid Miniprep Columns
• Monarch® DNA Elution Buffer
• Monarch® Plasmid Wash Buffer 2
• Monarch® Plasmid Wash Buffer 1
• Monarch® Plasmid Neutralization Buffer (B3)

1. Monarch® Plasmid DNA Miniprep Kit Protocol (NEB #T1010)

• Dos and Don'ts of Plasmid Minipreps
• Usage Guidelines for the Monarch Plasmid Miniprep Kit (#T1010) When Working with Low Copy Plasmids

1. Are plasmids recovered using the Monarch Plasmid Miniprep Kit endotoxin free?
2. I have used 5 ml of cell culture for plasmid isolation with the Monarch Plasmid Miniprep kit and I am obtaining low amounts of plasmid and/or contaminating gDNA. Why is this, and what are your suggestions to improve yield and purity?

• Troubleshooting Guide for DNA Cleanup and Plasmid Purification

• Monarch Nucleic Acid Purification Brochure

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• T1012L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• T1012L_v1_0101809
• T1012L_v1_10020882
• T1012L_v1_10029796
• T1012L_v1_10040685
• T1012L_v1_10126755
• T1012L_v1_10132565
• T1012L_v1_10135025
• T1012L_v1_10166557
• T1012L_v1_10175886
• T1012L_v1_10208631
• T1012L_v1_10240872

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Monarch^® Plasmid Lysis Buffer (B2)

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.